•Ten FMD virus mutants carrying deletion in NSP 3AB were engineered.•Largest deletion rescued was residues 87–144 in 3A along with first two copies of 3B.•Negative marker virus showed infectivity titre comparable to the parental virus.•An indirect ELISA targeting the deleted epitopes was developed.•3AB-truncated virus and its companion assay can be used in a marker vaccine strategy.
Virus Research – Elsevier
Published: Jan 2, 2018
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