Multiple events of gene manipulation via in pouch electroporation in a marsupial model of mammalian forebrain development

Multiple events of gene manipulation via in pouch electroporation in a marsupial model of... Article history: Background: The technique of in utero electroporation has been widely used in eutherians, such as mice Received 8 July 2017 and rats, to investigate brain development by selectively manipulating gene expression in specific neu- Received in revised form 6 September 2017 ronal populations. A major challenge, however, is that surgery is required to access the embryos, affecting Accepted 12 September 2017 animal survival and limiting the number of times it can be performed within the same litter. Available online 14 September 2017 New method: Marsupials are born at an early stage of brain development as compared to eutherians. Forebrain neurogenesis occurs mostly postnatally, allowing electroporation to be performed while joeys Keywords: develop attached to the teat. Here we describe the method of in pouch electroporation using the Australian Animal model marsupial fat-tailed dunnart (Sminthopsis crassicaudata, Dasyuridae). Brain development Results: In pouch electroporation is minimally invasive, quick, successful and anatomically precise. More- Brain evolution over, as no surgery is required, it can be performed several times in the same individual, and littermates Electroporation can undergo independent treatments. Neuronal labelling Transgenesis Comparison with existing method: As compared to in utero electroporation in rodents, in pouch electro- poration in marsupials http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Methods Elsevier

Multiple events of gene manipulation via in pouch electroporation in a marsupial model of mammalian forebrain development

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Publisher
Elsevier
Copyright
Copyright © 2017 The Authors
ISSN
0165-0270
eISSN
1872-678X
D.O.I.
10.1016/j.jneumeth.2017.09.004
Publisher site
See Article on Publisher Site

Abstract

Article history: Background: The technique of in utero electroporation has been widely used in eutherians, such as mice Received 8 July 2017 and rats, to investigate brain development by selectively manipulating gene expression in specific neu- Received in revised form 6 September 2017 ronal populations. A major challenge, however, is that surgery is required to access the embryos, affecting Accepted 12 September 2017 animal survival and limiting the number of times it can be performed within the same litter. Available online 14 September 2017 New method: Marsupials are born at an early stage of brain development as compared to eutherians. Forebrain neurogenesis occurs mostly postnatally, allowing electroporation to be performed while joeys Keywords: develop attached to the teat. Here we describe the method of in pouch electroporation using the Australian Animal model marsupial fat-tailed dunnart (Sminthopsis crassicaudata, Dasyuridae). Brain development Results: In pouch electroporation is minimally invasive, quick, successful and anatomically precise. More- Brain evolution over, as no surgery is required, it can be performed several times in the same individual, and littermates Electroporation can undergo independent treatments. Neuronal labelling Transgenesis Comparison with existing method: As compared to in utero electroporation in rodents, in pouch electro- poration in marsupials

Journal

Journal of Neuroscience MethodsElsevier

Published: Jan 1, 2018

References

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