MRC Image Processing Programs

MRC Image Processing Programs Digital image processing is an essential step in the determination of macromolecular structures by electron microscopy. Centrally important procedures are the averaging of many images of the subunit to improve the signal, the correction for various transfer functions, and the generation of a three-dimensional map from a set of two-dimensional projections. The detailed way in which these computational procedures are best carried out depends on the symmetry of the object and the type of specimen preparation. Over many years a large set of programs has been written by various members of the Laboratory of Molecular Biology for processing images of two-dimensional crystals and of particles with helical or icosahedral symmetry. The philosophy has been to write stand-alone programs and the whole system is given coherence by the adoption of standard formats for the storage and interchange of different kinds of data. This paper describes the current state of the programs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Structural Biology Elsevier

Loading next page...
 
/lp/elsevier/mrc-image-processing-programs-VI89JE1jpF
Publisher
Elsevier
Copyright
Copyright © 1996 Academic Press
ISSN
1047-8477
eISSN
1095-8657
DOI
10.1006/jsbi.1996.0003
pmid
8742717
Publisher site
See Article on Publisher Site

Abstract

Digital image processing is an essential step in the determination of macromolecular structures by electron microscopy. Centrally important procedures are the averaging of many images of the subunit to improve the signal, the correction for various transfer functions, and the generation of a three-dimensional map from a set of two-dimensional projections. The detailed way in which these computational procedures are best carried out depends on the symmetry of the object and the type of specimen preparation. Over many years a large set of programs has been written by various members of the Laboratory of Molecular Biology for processing images of two-dimensional crystals and of particles with helical or icosahedral symmetry. The philosophy has been to write stand-alone programs and the whole system is given coherence by the adoption of standard formats for the storage and interchange of different kinds of data. This paper describes the current state of the programs.

Journal

Journal of Structural BiologyElsevier

Published: Jan 1, 1996

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off