•It is shown that pepsin activity can be accurately determined using pH-stat at pH≤3.•This was used to monitor the gastric proteolysis of a model food (Infogest protocol).•The approach was then extended to a gastric digestion with an acidifying kinetics.•Degrees of hydrolysis were similar after 2h of digestion with both protocols.•The proposed approach is suitable for complex foods, not only protein solutions.
Food Chemistry – Elsevier
Published: Jan 15, 2018
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