Molecular methods for the differentiation of species used in production of cod-fish can detect commercial frauds

Molecular methods for the differentiation of species used in production of cod-fish can detect... A molecular approach was used to differentiate eight species commonly used in the production of cod-fish. Since visual identification can only be applied easily on whole fish, we used the PCR method to obtain a short fragment of the cytochrome B ( cytB ) gene that was then analyzed by RFLP, SSCP and DGGE. While RFLP and SSCP resulted in differentiation of only some of the species tested, DGGE was able to produce patterns that made possible the identification of the species considered. The application of molecular methods to the identification of the species in this study was found to be useful, fast and reliable. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food Control Elsevier

Molecular methods for the differentiation of species used in production of cod-fish can detect commercial frauds

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Publisher
Elsevier
Copyright
Copyright © 2003 Elsevier Ltd
ISSN
0956-7135
eISSN
1873-7129
D.O.I.
10.1016/j.foodcont.2003.11.003
Publisher site
See Article on Publisher Site

Abstract

A molecular approach was used to differentiate eight species commonly used in the production of cod-fish. Since visual identification can only be applied easily on whole fish, we used the PCR method to obtain a short fragment of the cytochrome B ( cytB ) gene that was then analyzed by RFLP, SSCP and DGGE. While RFLP and SSCP resulted in differentiation of only some of the species tested, DGGE was able to produce patterns that made possible the identification of the species considered. The application of molecular methods to the identification of the species in this study was found to be useful, fast and reliable.

Journal

Food ControlElsevier

Published: Jan 1, 2005

References

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