Molecular interaction of triclosan with superoxide dismutase (SOD) reveals a potentially toxic mechanism of the antimicrobial agent

Molecular interaction of triclosan with superoxide dismutase (SOD) reveals a potentially toxic... In this article, the interaction mechanism between the superoxide dismutase (SOD) and the triclosan (TCS), a kind of antimicrobial agent which is of widely application with potential effects both on environment and human health, was explored through a series of spectroscopic methods, animal experiment and the molecular docking simulation. The negative free energy change ∆G, enthalpy change (∆H = 162.21 kJmol−1) and entropy change (∆S = 615 Jmol−1K−1) demonstrated that TCS could combine with SOD spontaneously through hydrophobic interaction to form a complex. The binding constants of Ka293 and Ka313 were 1.706 × 103 and 1.2 × 105 Lmol−1, respectively. Furthermore, the interaction could also influence the skeleton structure and secondary contents of SOD. The molecular docking analysis revealed the TCS located between two subunits of SOD, and there was a hydrogen bond between TCS and the residue Asn51 of SOD, which influenced the structure of protein and resulted in a decrease of enzyme activity. This work could help understand the interaction mechanism between SOD and TCS. Moreover, it could also be used to consult for toxicity assessment of TCS at molecular level. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Ecotoxicology and Environmental Safety Elsevier

Molecular interaction of triclosan with superoxide dismutase (SOD) reveals a potentially toxic mechanism of the antimicrobial agent

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Inc.
ISSN
0147-6513
eISSN
1090-2414
D.O.I.
10.1016/j.ecoenv.2018.01.055
Publisher site
See Article on Publisher Site

Abstract

In this article, the interaction mechanism between the superoxide dismutase (SOD) and the triclosan (TCS), a kind of antimicrobial agent which is of widely application with potential effects both on environment and human health, was explored through a series of spectroscopic methods, animal experiment and the molecular docking simulation. The negative free energy change ∆G, enthalpy change (∆H = 162.21 kJmol−1) and entropy change (∆S = 615 Jmol−1K−1) demonstrated that TCS could combine with SOD spontaneously through hydrophobic interaction to form a complex. The binding constants of Ka293 and Ka313 were 1.706 × 103 and 1.2 × 105 Lmol−1, respectively. Furthermore, the interaction could also influence the skeleton structure and secondary contents of SOD. The molecular docking analysis revealed the TCS located between two subunits of SOD, and there was a hydrogen bond between TCS and the residue Asn51 of SOD, which influenced the structure of protein and resulted in a decrease of enzyme activity. This work could help understand the interaction mechanism between SOD and TCS. Moreover, it could also be used to consult for toxicity assessment of TCS at molecular level.

Journal

Ecotoxicology and Environmental SafetyElsevier

Published: May 30, 2018

References

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