Molecular cloning of an ester-forming triterpenoid: UDP-glucose 28-O-glucosyltransferase involved in saponin biosynthesis from the medicinal plant Centella asiatica

Molecular cloning of an ester-forming triterpenoid: UDP-glucose 28-O-glucosyltransferase involved... Plant Science 262 (2017) 9–17 Contents lists available at ScienceDirect Plant Science journal homepage: www.elsevier.com/locate/plantsci Molecular cloning of an ester-forming triterpenoid: UDP-glucose 28-O- MARK glucosyltransferase involved in saponin biosynthesis from the medicinal plant Centella asiatica a,b,1 a,1 a,c a a Fernanda de Costa , Carla J.S. Barber , Yeon-bok Kim , Darwin W. Reed , Haixia Zhang , b a, Arthur G. Fett-Neto , Patrick S. Covello National Research Council of Canada, 110 Gymnasium Pl., Saskatoon, SK S7N 0W9, Canada Plant Physiology Laboratory, Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Porto Alegre, RS 91501-970, Brazil Herbal Crop Research Division, Department of Herbal Crop Research, Bisanro 92, Eumseong, Chungbuk, 369-873, Korea ARTICLE I NFO ABSTRACT Keywords: Triterpene saponins include bioactive compounds with structures consisting of triterpene aglycones (sapogenins) Centella asiatica and one or more sugar moieties linked through acetal or ester glycosidic linkages at one or more sites. Centella Apiaceae asiatica (L.) Urban is a medicinal plant that contains bioactive ursane-type saponins, such as madecassoside and Saponin biosynthesis asiaticoside. In this work, glucosylation of triterpenoids in C. asiatica was investigated starting with plant ex- Glucosyltransferase tracts. An enzyme http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Science Elsevier

Molecular cloning of an ester-forming triterpenoid: UDP-glucose 28-O-glucosyltransferase involved in saponin biosynthesis from the medicinal plant Centella asiatica

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0168-9452
D.O.I.
10.1016/j.plantsci.2017.05.009
Publisher site
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Abstract

Plant Science 262 (2017) 9–17 Contents lists available at ScienceDirect Plant Science journal homepage: www.elsevier.com/locate/plantsci Molecular cloning of an ester-forming triterpenoid: UDP-glucose 28-O- MARK glucosyltransferase involved in saponin biosynthesis from the medicinal plant Centella asiatica a,b,1 a,1 a,c a a Fernanda de Costa , Carla J.S. Barber , Yeon-bok Kim , Darwin W. Reed , Haixia Zhang , b a, Arthur G. Fett-Neto , Patrick S. Covello National Research Council of Canada, 110 Gymnasium Pl., Saskatoon, SK S7N 0W9, Canada Plant Physiology Laboratory, Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Porto Alegre, RS 91501-970, Brazil Herbal Crop Research Division, Department of Herbal Crop Research, Bisanro 92, Eumseong, Chungbuk, 369-873, Korea ARTICLE I NFO ABSTRACT Keywords: Triterpene saponins include bioactive compounds with structures consisting of triterpene aglycones (sapogenins) Centella asiatica and one or more sugar moieties linked through acetal or ester glycosidic linkages at one or more sites. Centella Apiaceae asiatica (L.) Urban is a medicinal plant that contains bioactive ursane-type saponins, such as madecassoside and Saponin biosynthesis asiaticoside. In this work, glucosylation of triterpenoids in C. asiatica was investigated starting with plant ex- Glucosyltransferase tracts. An enzyme

Journal

Plant ScienceElsevier

Published: Sep 1, 2017

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