Microfluidic techniques for high throughput single cell analysis

Microfluidic techniques for high throughput single cell analysis Available online at www.sciencedirect.com ScienceDirect Microfluidic techniques for high throughput single cell analysis Amy Reece, Bingzhao Xia, Zhongliang Jiang, Benjamin Noren, Ralph McBride and John Oakey The microfabrication of microfluidic control systems and the and their environment [7]. As increasingly sensitive mo- development of increasingly sensitive molecular amplification lecular biology tools have been introduced, a new class of tools have enabled the miniaturization of single cells analytical lab-on-a-chip devices, capable of sorting, segmenting, and platforms. Only recently has the throughput of these platforms individually characterizing large numbers of single cells, increased to a level at which populations can be screened at has emerged [8]. the single cell level. Techniques based upon both active and passive manipulation are now capable of discriminating The increasing throughput of microfluidic single cell between single cell phenotypes for sorting, diagnostic or analysis technologies has enabled cellular populations prognostic applications in a variety of clinical scenarios. The to be characterized and parsed for heterogeneity [9], introduction of multiphase microfluidics enables the diseased or mutant cells to be isolated [10], and rare cells segmentation of single cells into biochemically discrete to be selected from complex cellular backgrounds [8]. picoliter environments. The combination of these techniques Techniques http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Opinion in Biotechnology Elsevier

Microfluidic techniques for high throughput single cell analysis

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Publisher
Elsevier
Copyright
Copyright © 2016 Elsevier Ltd
ISSN
0958-1669
D.O.I.
10.1016/j.copbio.2016.02.015
Publisher site
See Article on Publisher Site

Abstract

Available online at www.sciencedirect.com ScienceDirect Microfluidic techniques for high throughput single cell analysis Amy Reece, Bingzhao Xia, Zhongliang Jiang, Benjamin Noren, Ralph McBride and John Oakey The microfabrication of microfluidic control systems and the and their environment [7]. As increasingly sensitive mo- development of increasingly sensitive molecular amplification lecular biology tools have been introduced, a new class of tools have enabled the miniaturization of single cells analytical lab-on-a-chip devices, capable of sorting, segmenting, and platforms. Only recently has the throughput of these platforms individually characterizing large numbers of single cells, increased to a level at which populations can be screened at has emerged [8]. the single cell level. Techniques based upon both active and passive manipulation are now capable of discriminating The increasing throughput of microfluidic single cell between single cell phenotypes for sorting, diagnostic or analysis technologies has enabled cellular populations prognostic applications in a variety of clinical scenarios. The to be characterized and parsed for heterogeneity [9], introduction of multiphase microfluidics enables the diseased or mutant cells to be isolated [10], and rare cells segmentation of single cells into biochemically discrete to be selected from complex cellular backgrounds [8]. picoliter environments. The combination of these techniques Techniques

Journal

Current Opinion in BiotechnologyElsevier

Published: Aug 1, 2016

References

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