Microfluidic techniques for high throughput single cell analysis

Microfluidic techniques for high throughput single cell analysis Available online at www.sciencedirect.com ScienceDirect Microfluidic techniques for high throughput single cell analysis Amy Reece, Bingzhao Xia, Zhongliang Jiang, Benjamin Noren, Ralph McBride and John Oakey The microfabrication of microfluidic control systems and the and their environment [7]. As increasingly sensitive mo- development of increasingly sensitive molecular amplification lecular biology tools have been introduced, a new class of tools have enabled the miniaturization of single cells analytical lab-on-a-chip devices, capable of sorting, segmenting, and platforms. Only recently has the throughput of these platforms individually characterizing large numbers of single cells, increased to a level at which populations can be screened at has emerged [8]. the single cell level. Techniques based upon both active and passive manipulation are now capable of discriminating The increasing throughput of microfluidic single cell between single cell phenotypes for sorting, diagnostic or analysis technologies has enabled cellular populations prognostic applications in a variety of clinical scenarios. The to be characterized and parsed for heterogeneity [9], introduction of multiphase microfluidics enables the diseased or mutant cells to be isolated [10], and rare cells segmentation of single cells into biochemically discrete to be selected from complex cellular backgrounds [8]. picoliter environments. The combination of these techniques Techniques http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Opinion in Biotechnology Elsevier

Microfluidic techniques for high throughput single cell analysis

Loading next page...
 
/lp/elsevier/microfluidic-techniques-for-high-throughput-single-cell-analysis-dlIKuN3oDY
Publisher
Elsevier
Copyright
Copyright © 2016 Elsevier Ltd
ISSN
0958-1669
D.O.I.
10.1016/j.copbio.2016.02.015
Publisher site
See Article on Publisher Site

Abstract

Available online at www.sciencedirect.com ScienceDirect Microfluidic techniques for high throughput single cell analysis Amy Reece, Bingzhao Xia, Zhongliang Jiang, Benjamin Noren, Ralph McBride and John Oakey The microfabrication of microfluidic control systems and the and their environment [7]. As increasingly sensitive mo- development of increasingly sensitive molecular amplification lecular biology tools have been introduced, a new class of tools have enabled the miniaturization of single cells analytical lab-on-a-chip devices, capable of sorting, segmenting, and platforms. Only recently has the throughput of these platforms individually characterizing large numbers of single cells, increased to a level at which populations can be screened at has emerged [8]. the single cell level. Techniques based upon both active and passive manipulation are now capable of discriminating The increasing throughput of microfluidic single cell between single cell phenotypes for sorting, diagnostic or analysis technologies has enabled cellular populations prognostic applications in a variety of clinical scenarios. The to be characterized and parsed for heterogeneity [9], introduction of multiphase microfluidics enables the diseased or mutant cells to be isolated [10], and rare cells segmentation of single cells into biochemically discrete to be selected from complex cellular backgrounds [8]. picoliter environments. The combination of these techniques Techniques

Journal

Current Opinion in BiotechnologyElsevier

Published: Aug 1, 2016

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial