Method of derivation and differentiation of mouse embryonic stem cells generating synchronous neuronal networks

Method of derivation and differentiation of mouse embryonic stem cells generating synchronous... •A refinement of a protocol for mouse ES cell differentiation is described.•ES cell derived and primary neuron cultures were grown and functionally analysed using microelectrode arrays.•At 35 days in culture ES cell differentiated neuronal networks recapitulated functional properties of primary cortical neuronal networks.•ES cell neurons may serve as a viable alternative to primary neurons with implications for modelling of human disease. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Methods Elsevier

Method of derivation and differentiation of mouse embryonic stem cells generating synchronous neuronal networks

Loading next page...
 
/lp/elsevier/method-of-derivation-and-differentiation-of-mouse-embryonic-stem-cells-PdQ08s0bff
Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0165-0270
eISSN
1872-678X
D.O.I.
10.1016/j.jneumeth.2017.08.018
Publisher site
See Article on Publisher Site

Abstract

•A refinement of a protocol for mouse ES cell differentiation is described.•ES cell derived and primary neuron cultures were grown and functionally analysed using microelectrode arrays.•At 35 days in culture ES cell differentiated neuronal networks recapitulated functional properties of primary cortical neuronal networks.•ES cell neurons may serve as a viable alternative to primary neurons with implications for modelling of human disease.

Journal

Journal of Neuroscience MethodsElsevier

Published: Jan 1, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off