Method for simple and rapid concentration of Zika virus particles from infected cell-culture supernatants

Method for simple and rapid concentration of Zika virus particles from infected cell-culture... Experimental studies on Zika virus (ZIKV) may require improvement of infectious titers in viral stocks obtained by cell culture amplification. The use of centrifugal filter devices to increase infectious titers of ZIKV from cell-culture supernatants is highlighted here. A mean gain of 2.33 ± 0.12 log10 DICT50/mL was easily and rapidly obtained with this process. This efficient method of ultrafiltration may be applied to other viruses and be useful in various experimental studies requiring high viral titers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

Method for simple and rapid concentration of Zika virus particles from infected cell-culture supernatants

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2018.02.014
Publisher site
See Article on Publisher Site

Abstract

Experimental studies on Zika virus (ZIKV) may require improvement of infectious titers in viral stocks obtained by cell culture amplification. The use of centrifugal filter devices to increase infectious titers of ZIKV from cell-culture supernatants is highlighted here. A mean gain of 2.33 ± 0.12 log10 DICT50/mL was easily and rapidly obtained with this process. This efficient method of ultrafiltration may be applied to other viruses and be useful in various experimental studies requiring high viral titers.

Journal

Journal of Virological MethodsElsevier

Published: May 1, 2018

References

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