Bioresource Technology 214 (2016) 528–533 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech Metallic/bimetallic magnetic nanoparticle functionalization for immobilization of a-amylase for enhanced reusability in bio-catalytic processes a a a a a c Vishal Singh , Kanak Rakshit , Shweta Rathee , Stanzin Angmo , Shimayali Kaushal , Pankaj Garg , c d b a,⇑ Jong Hoon Chung , Rajat Sandhir , Rajender S. Sangwan , Nitin Singhal National Agri-food Biotechnology Institute (NABI), C-127, I Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India Center for Innovative and Applied Bioprocessing (CIAB), C-127, II Floor, Phase-VIII, Industrial Area, SAS Nagar, Mohali 160071, Punjab, India Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea Department of Biochemistry, Panjab University, Chandigarh 160014, India highlights graphical a bstract Bimetallic based magnetic nanoparticles have been developed to immobilize the enzyme. Enhanced reusability in bio-catalytic processes has been discussed. Activity and kinetic parameters have been calculated and compared. The properties of immobilized amylase were enhanced than the free enzyme. ar ti c l e i nf o ab stra ct Article history: Novel magnetic nanoparticles coated with silica and gold were synthesized for immobilization of
Bioresource Technology – Elsevier
Published: Aug 1, 2016
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