Metabolic profiling of Gegenqinlian decoction in rat plasma, urine, bile and feces after oral administration by ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry

Metabolic profiling of Gegenqinlian decoction in rat plasma, urine, bile and feces after oral... Gegenqinlian decoction (GQLD) as a traditional Chinese medical (TCM) prescription is made from four Chinese herbs and has been used for centuries to treat pyrexia and diarrhea. In this work, an ultra-high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was established to profile the metabolic fate of GQLD in rat plasma, urine, bile and feces. The analysis was performed on a Waters Acquity UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) at 35 °C within 25 min. A total of 174 compounds mainly including flavonoids, alkaloids and triterpenoids in rat plasma, urine, bile and feces after oral administration of GQLD were identified or tentatively characterized by comparison with retention times, accurate mass within 5 ppm error and their characteristic MS fragmentation ions. Among compounds in rats, 107 compounds are prototypes and 67 compounds are metabolites. Results demonstrated that metabolic pathways of GQLD in rats included methylation, demethylation, hydrogenation, hydrolysis, hydroxylation, methoxylation, sulfate conjugation and glucuronide conjugation. A clear understanding of the absorption and metabolism of GQLD is very important in its rational clinical use and pharmacological research. These findings enhance our understanding of the metabolism and effective forms of GQLD. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Chromatography B Elsevier

Metabolic profiling of Gegenqinlian decoction in rat plasma, urine, bile and feces after oral administration by ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry

Loading next page...
 
/lp/elsevier/metabolic-profiling-of-gegenqinlian-decoction-in-rat-plasma-urine-bile-Ejkfia42k6
Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
1570-0232
eISSN
1873-376X
D.O.I.
10.1016/j.jchromb.2018.02.001
Publisher site
See Article on Publisher Site

Abstract

Gegenqinlian decoction (GQLD) as a traditional Chinese medical (TCM) prescription is made from four Chinese herbs and has been used for centuries to treat pyrexia and diarrhea. In this work, an ultra-high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was established to profile the metabolic fate of GQLD in rat plasma, urine, bile and feces. The analysis was performed on a Waters Acquity UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) at 35 °C within 25 min. A total of 174 compounds mainly including flavonoids, alkaloids and triterpenoids in rat plasma, urine, bile and feces after oral administration of GQLD were identified or tentatively characterized by comparison with retention times, accurate mass within 5 ppm error and their characteristic MS fragmentation ions. Among compounds in rats, 107 compounds are prototypes and 67 compounds are metabolites. Results demonstrated that metabolic pathways of GQLD in rats included methylation, demethylation, hydrogenation, hydrolysis, hydroxylation, methoxylation, sulfate conjugation and glucuronide conjugation. A clear understanding of the absorption and metabolism of GQLD is very important in its rational clinical use and pharmacological research. These findings enhance our understanding of the metabolism and effective forms of GQLD.

Journal

Journal of Chromatography BElsevier

Published: Mar 15, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off