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production [21]. To address the discrepancy between the results obtained with the native oriRi base vector [9] and the pTF::Ri [21] multi-copy vector, we sequenced the high copy plasmid pTF::Ri. Sequence ...
of plasmids and changes of fluorescence in dividing cells were also modelled using MATLAB. MATLAB code for all models is available at https://github.com/alex297/ model -of-binary-counter- based -on-recombination ...
as the standard M9 medium , except NH4Cl was omitted and the final concentration of NaCl final was increased to 1.5 g/L. Plasmids and strains The strains, plasmids , primers and double stranded DNA fragments used ...
(locations designated in Figure 3) amplified a 10-kb fragment when mitochondrial DNA templates from NEP-2 and POP were used. Under the same conditions, no amplification product was detected with CMS-Sprite ...
manipulation and plasmid preparation. Plasmid pYES2 (Invitrogen®) was used as the vector for cloning, DNA manipulation, and protein expression. A. kawachii was grown according to Contreras et al. [6]. One-liter ...
, the operation time of this method was shorter than that of the previous method, especially for repeated integration. This method, which is based on the mobilizable plasmid pK18-MBPMT, thus represents ...
plasmid in a given strain is denoted as [Cir+] or [Cir0], respectively. This designation does not include ARS- based or 2-micron-derived plasmid constructs. The genotype of a strain containing ...
of rapamycin production . First, a genome-scale metabolic model of Streptomyces hygroscopicus ATCC 29253 was constructed based on its annotated genome and biochemical information. The model consists of 1003 ...
SC-Ura+G418 liquid medium from which the plasmid was recovered using a Zymoprep Yeast Plasmid Miniprep II Kit (D2004; Zymo Research, Irvine, CA). The isolated plasmid was then transformed ...
to mispairing of DNA bases , which mostly results in GC→AT transitions and G/C→T/A transversions [9], in the long-run genetic drift is expected to increase the elements’ overall AT-content. On the other hand ...
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