Low-Dose Automated Electron Tomography: A Recent Implementation

Low-Dose Automated Electron Tomography: A Recent Implementation Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Structural Biology Elsevier

Low-Dose Automated Electron Tomography: A Recent Implementation

Journal of Structural Biology, Volume 120 (3) – Dec 1, 1997

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Publisher
Elsevier
Copyright
Copyright © 1997 Academic Press
ISSN
1047-8477
eISSN
1095-8657
DOI
10.1006/jsbi.1997.3924
pmid
9441926
Publisher site
See Article on Publisher Site

Abstract

Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made.

Journal

Journal of Structural BiologyElsevier

Published: Dec 1, 1997

References

  • SPIDER and WEB: Processing and visualization of images in 3D electron microscopy and related fields
    Frank, J.; Radermacher, M.; Penczek, P.; Zhu, J.; Li, Y.; Ladjadj, M.; Leith, A.

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