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Scholar Crossref Search ADS PubMed WorldCat 27 Gratama JW , Markendez P, Kraan J et al. Loss of CD34 + hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte ...
antigen was identified as a marker of hematopoietic stem and progenitor cells [18, 19]. A few years later, the clinical implication was documented by the Milan Group reporting that CD34 + cells circulate ...
progenitor fraction as CD43/45+ CD34 +, and our more primitive hematopoietic cell fraction, previously described as HSC-like [5], as CD43/45+ CD34 + CD90+. The cell permeable dye CellROX Deep Red, becoming ...
in enriched CD34 + cell fractions if related to underlying CD34 + cell numbers. Discussion While the total number of hematopoietic cells can be counted readily by cytofluorimetry due to their common surface ...
]. We have shown that Mef/Elf4, an ETS transcription factor, regulates murine HSC quiescence and that acute knockdown of MEF in human CB CD34 + cells also enhances hematopoietic stem/ progenitor cells ...
that concomitant CD34 and CD43 expression by hematopoietic progenitors is not a phenotype describing a differentiation state sufficient before engaging B cell differentiation. We rather propose advanced progenitors ...
and 14 DIV. Cells were harvested and washed with 1 ml PBS containing 2 mM EDTA and 5% HSA. 15 μl of FITC-conjugated anti-human CD45 and 15 μl of PE-conjugated anti-human CD34 antibody as well as 10 μl 7AAD ...
reconstitution in mice, induced the rapid appearance of CD34 + cells with a concomitant upregulation of mesoderm-related markers. Profiling of cord blood hematopoietic progenitor cell populations identified miR ...
and other blood cell disease. Conclusion Enrichment of human hematopoietic stem/ progenitor cells further than standard CD34 + cell preparations (e.g. CD34 +/CD38−) may facilitate gene therapy by reducing ...
]. CD34 + populations in bone marrow or blood samples are a hematopoietic stem/ progenitor mix, of which the majority of cells are progenitors [4]. Human, donor derived CD34 + hematopoietic stem and progenitor ...
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