Light sheet microscopy for real-time developmental biology

Light sheet microscopy for real-time developmental biology Within only a few short years, light sheet microscopy has contributed substantially to the emerging field of real-time developmental biology. Low photo-toxicity and high-speed multiview acquisition have made selective plane illumination microscopy (SPIM) a popular choice for studies of organ morphogenesis and function in zebrafish, Drosophila , and other model organisms. A multitude of different light sheet microscopes have emerged for the noninvasive imaging of specimens ranging from single molecules to cells, tissues, and entire embryos. In particular, developmental biology can benefit from the ability to watch developmental events occur in real time in an entire embryo, thereby advancing our understanding on how cells form tissues and organs. However, it presents a new challenge to our existing data and image processing tools. This review gives an overview of where we stand as light sheet microscopy branches out, explores new areas, and becomes more specialized. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Opinion in Genetics & Development Elsevier

Light sheet microscopy for real-time developmental biology

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Publisher
Elsevier
Copyright
Copyright © 2011 Elsevier Ltd
ISSN
0959-437x
D.O.I.
10.1016/j.gde.2011.09.009
Publisher site
See Article on Publisher Site

Abstract

Within only a few short years, light sheet microscopy has contributed substantially to the emerging field of real-time developmental biology. Low photo-toxicity and high-speed multiview acquisition have made selective plane illumination microscopy (SPIM) a popular choice for studies of organ morphogenesis and function in zebrafish, Drosophila , and other model organisms. A multitude of different light sheet microscopes have emerged for the noninvasive imaging of specimens ranging from single molecules to cells, tissues, and entire embryos. In particular, developmental biology can benefit from the ability to watch developmental events occur in real time in an entire embryo, thereby advancing our understanding on how cells form tissues and organs. However, it presents a new challenge to our existing data and image processing tools. This review gives an overview of where we stand as light sheet microscopy branches out, explores new areas, and becomes more specialized.

Journal

Current Opinion in Genetics & DevelopmentElsevier

Published: Oct 1, 2011

References

  • Optically sectioned imaging by oblique plane microscopy
    Dunsby, C.
  • Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy
    Keller, P.J.; Schmidt, A.D.; Wittbrodt, J.; Stelzer, E.H.K.
  • 4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies
    Swoger, J.; Muzzopappa, M.; López-Schier, H.; Sharpe, J.
  • Ultramicroscopy: 3D reconstruction of large microscopical specimens
    Becker, K.; Jährling, N.; Kramer, E.R.; Schnorrer, F.; Dodt, H.

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