Isolation and partial characterization of a novel porcine astrovirus

Isolation and partial characterization of a novel porcine astrovirus Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a diarrhoeal specimen. Furthermore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus, PAstV. Phylogenetic analysis clustered the novel isolate, Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Veterinary Microbiology Elsevier

Isolation and partial characterization of a novel porcine astrovirus

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Publisher
Elsevier
Copyright
Copyright © 2006 Elsevier B.V.
ISSN
0378-1135
eISSN
1873-2542
D.O.I.
10.1016/j.vetmic.2006.06.020
Publisher site
See Article on Publisher Site

Abstract

Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a diarrhoeal specimen. Furthermore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus, PAstV. Phylogenetic analysis clustered the novel isolate, Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses.

Journal

Veterinary MicrobiologyElsevier

Published: Oct 31, 2006

References

  • Astrovirus epidemiologically linked to pre-weaning diarrhoea in mink
    Englund, L.; Chriel, M.; Dietz, H.H.; Hedlund, K.O.
  • Typing of porcine circovirus in clinical specimens by multiplex PCR
    Larochelle, R.; Antaya, M.; Morin, M.; Magar, R.

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