Involvement of Fas and FasL in Ectromelia virus-induced apoptosis in mouse brain

Involvement of Fas and FasL in Ectromelia virus-induced apoptosis in mouse brain In this study we showed that the virulent Moscow strain of Ectromelia virus (ECTV-MOS) infection leads to induction of apoptosis in the BALB/c mouse central nervous system. ECTV-MOS-infected cells and inflammation sites were found in brain parenchyma between 5 and 15 days after footpad infection with ECTV-MOS. Infected cells consisted of microglia and monocytes, astrocytes and oligodendrocytes and these type of cells underwent apoptosis within 5–15 days post infection (d.p.i.). The highest number of apoptotic cells was found at 5 and 10 d.p.i. and represented mainly microglia (61.4% and 38.6% of apoptotic cells, respectively) and astrocytes (21% and 8.9%, respectively). The number of apoptotic oligodendrocytes was 5.4% and 4.5%, respectively. Fluorometric assays demonstrated involvement of caspase-1, -3 and -8 but not caspase-9 in apoptosis in ECTV-MOS-infected mouse brains. Expression of Fas/FasL was significantly increased on ECTV-MOS-infected cells between 5 and 15 d.p.i., whereas Fas was up-regulated also on the surrounding, non-infected cells. Taking together we may conclude that ECTV-MOS infection of microglia and astrocytes leads to local inflammation resulting in Fas/FasL up-regulation and apoptosis, which limits mouse central nervous system infection with ECTV-MOS. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Research Elsevier

Involvement of Fas and FasL in Ectromelia virus-induced apoptosis in mouse brain

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Publisher
Elsevier
Copyright
Copyright © 2005 Elsevier B.V.
ISSN
0168-1702
D.O.I.
10.1016/j.virusres.2005.08.001
Publisher site
See Article on Publisher Site

Abstract

In this study we showed that the virulent Moscow strain of Ectromelia virus (ECTV-MOS) infection leads to induction of apoptosis in the BALB/c mouse central nervous system. ECTV-MOS-infected cells and inflammation sites were found in brain parenchyma between 5 and 15 days after footpad infection with ECTV-MOS. Infected cells consisted of microglia and monocytes, astrocytes and oligodendrocytes and these type of cells underwent apoptosis within 5–15 days post infection (d.p.i.). The highest number of apoptotic cells was found at 5 and 10 d.p.i. and represented mainly microglia (61.4% and 38.6% of apoptotic cells, respectively) and astrocytes (21% and 8.9%, respectively). The number of apoptotic oligodendrocytes was 5.4% and 4.5%, respectively. Fluorometric assays demonstrated involvement of caspase-1, -3 and -8 but not caspase-9 in apoptosis in ECTV-MOS-infected mouse brains. Expression of Fas/FasL was significantly increased on ECTV-MOS-infected cells between 5 and 15 d.p.i., whereas Fas was up-regulated also on the surrounding, non-infected cells. Taking together we may conclude that ECTV-MOS infection of microglia and astrocytes leads to local inflammation resulting in Fas/FasL up-regulation and apoptosis, which limits mouse central nervous system infection with ECTV-MOS.

Journal

Virus ResearchElsevier

Published: Feb 1, 2006

References

  • Measles virus infection of cerebral endothelial cells and effect on their adhesive properties
    Cosby, S.L.; Brankin, B.
  • Involvement of the CD95 (APO-1/Fas) receptor/ligand system in multiple sclerosis brain
    Dowling, P.; Shang, G.; Raval, S.; Menonna, J.; Cook, S.; Husar, W.
  • Mousepox
    Fenner, F.R.; Buller, M.L.
  • Apoptosis and neurologic disease
    Honing, L.S.; Rosenberg, R.N.
  • The central effectors of cell death in the immune system
    Rathmell, J.C.; Thompson, C.B.
  • Apoptosis and cancer: strategies for integrating programmed cell death
    Reed, C.J.
  • Astrocyte activation and apoptosis: their roles in the neuropathology of HIV Infection
    Sabri, F.; Titanji, K.; De Milito, A.; Chiodi, F.

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