The activity and stability of enzymes in supercritical carbon dioxide fluid are the crucial points and basis for developing and applying green, environmentally friendly processes and/or reactions in this water free media in different industries, which has attracted increasing interesting recently. The objective of the present work is to investigate the activity and stability of pectinase in supercritical carbon dioxide media, as well as for its structure and conformation changes. The results show that the activity and stability of pectinase were significantly improved under appropriate conditions. Significant increases in activity and stability of treated pectinase could be available with pressure lower than 15.0 MPa, whereas, temperature tends to reduce enzymatic activity and stability. An excellent stability of pectinase with improved activity was observed with duration from 0.5 h to 4.0 h. Fourier transfer infrared spectra, ultraviolet spectra, fluorescence spectra and scanning electron microscopy analyses indicate that alterations in the secondary and tertiary structures, and morphology of treated pectinase, were occurred without conspicuous changes in its primary structure. An exposure of the residual side aromatic groups of tryptophan on polypeptide to outer surface of the enzyme in solution was also detected. Therefore, all the investigations further demonstrate that the supercritical treatment is an efficient method to improve the activity and stability of the enzyme due to conformational changes, and there is also a feasible to perform cleaner and sustainable production processes or reactions with pectinase in supercritical carbon dioxide media.
Journal of Cleaner Production – Elsevier
Published: Jul 1, 2016
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera