Intraperitoneal administration of thioredoxin decreases brain damage from ischemic stroke

Intraperitoneal administration of thioredoxin decreases brain damage from ischemic stroke Recent studies demonstrate that Thioredixin (Trx) possesses a neuronal protective effect and closely relates to oxidative stress and apoptosis of cerebral ischemia injury. The present study was conducted to validate the neuroprotective effect of recombinant human Trx-1 (rhTrx-1) and its potential mechanisms against ischemia injury at middle cerebral artery occlusion (MCAO) in mice. rhTrx-1 was administrated intraperitoneally at a dose of 5, 10 and 20mg/kg 30min before MCAO in mice, and its neuronal protective effect was evaluated by neurological deficit score, brain dry–wet weight, 2,3,5-triphenyltetrazolium chloride (TTC) staining. The protein carbonyl content and HO-1 were detected to investigate its potential anti-oxidative and anti-inflammatory property, and the anti-apoptotic ability of rhTrx-1 was assessed by casepase-3 and TUNEL staining. The results demonstrated that rhTrx-1 significantly improved neurological functions and reduced cerebral infarction and apoptotic cell death at 24h after MCAO. Moreover, rhTrx-1 resulted in a significant decrease in carbonyl contents and HO-1 against oxidative stress, which turned to be fast reduction during the first 24h and tended to be stable from 24h to 72h after MCAO. The study shows that rhTrx-1 exerts an neuroprotective effect in cerebral ischemia injury. The anti-oxidative, anti-apoptotic and anti-inflammatory properties of rhTrx-1 are more likely to succeed as a therapeutic approach to diminish oxidative stress-induced neuronal apoptotic cell death in acute ischemic stroke. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Intraperitoneal administration of thioredoxin decreases brain damage from ischemic stroke

Loading next page...
 
/lp/elsevier/intraperitoneal-administration-of-thioredoxin-decreases-brain-damage-P0HivBKCsU
Publisher
Elsevier
Copyright
Copyright © 2015 Elsevier B.V.
ISSN
0006-8993
D.O.I.
10.1016/j.brainres.2015.04.033
Publisher site
See Article on Publisher Site

Abstract

Recent studies demonstrate that Thioredixin (Trx) possesses a neuronal protective effect and closely relates to oxidative stress and apoptosis of cerebral ischemia injury. The present study was conducted to validate the neuroprotective effect of recombinant human Trx-1 (rhTrx-1) and its potential mechanisms against ischemia injury at middle cerebral artery occlusion (MCAO) in mice. rhTrx-1 was administrated intraperitoneally at a dose of 5, 10 and 20mg/kg 30min before MCAO in mice, and its neuronal protective effect was evaluated by neurological deficit score, brain dry–wet weight, 2,3,5-triphenyltetrazolium chloride (TTC) staining. The protein carbonyl content and HO-1 were detected to investigate its potential anti-oxidative and anti-inflammatory property, and the anti-apoptotic ability of rhTrx-1 was assessed by casepase-3 and TUNEL staining. The results demonstrated that rhTrx-1 significantly improved neurological functions and reduced cerebral infarction and apoptotic cell death at 24h after MCAO. Moreover, rhTrx-1 resulted in a significant decrease in carbonyl contents and HO-1 against oxidative stress, which turned to be fast reduction during the first 24h and tended to be stable from 24h to 72h after MCAO. The study shows that rhTrx-1 exerts an neuroprotective effect in cerebral ischemia injury. The anti-oxidative, anti-apoptotic and anti-inflammatory properties of rhTrx-1 are more likely to succeed as a therapeutic approach to diminish oxidative stress-induced neuronal apoptotic cell death in acute ischemic stroke.

Journal

Brain ResearchElsevier

Published: Jul 30, 2015

References

  • Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats
    Amemiya, S.; Kamiya, T.; Nito, C.; Inaba, T.; Kato, K.; Ueda, M.; Shimazaki, K.; Katayama, Y.
  • Oxygen radicals in focal cerebral ischemia
    Chan, P.H.
  • Thioredoxin, a singlet oxygen quencher and hydroxyl radical scavenger: redox independent functions
    Das, K.C.; Das, C.K.
  • Postischemic infusion of Cu/Zn superoxide dismutase or SOD:Tet451 reduces cerebral infarction following focal ischemia/reperfusion in rats
    Francis, J.W.; Ren, J.; Warren, L.; Brown, R.H.; Finklestein, S.P.
  • Thioredoxin and glutaredoxin systems
    Holmgren, A.
  • Thioredoxin-1 attenuates post-ischemic neuronal apoptosis via reducing oxidative/nitrative stress
    Ma, Y.H.; Su, N.; Chao, X.D.; Zhang, Y.Q.; Zhang, L.; Han, F.; Luo, P.; Fei, Z.; Qu, Y.
  • Oxidative stress and neuronal death/survival signaling in cerebral ischemia
    Saito, A.; Maier, C.M.; Narasimhan, P.; Nishi, T.; Song, Y.S.; Yu, F.; Liu, J.; Lee, Y.S.; Nito, C.; Kamada, H.; Dodd, R.L.; Hsieh, L.B.; Hassid, B.; Kim, E.E.; Gonzalez, M.; Chan, P.H.
  • Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1
    Saitoh, M.; Nishitoh, H.; Fujii, M.; Takeda, K.; Tobiume, K.; Sawada, Y.; Kawabata, M.; Miyazono, K.; Ichijo, H.
  • Attenuation of neuronal degeneration in thioredoxin-1 overexpressing mice after mild focal ischemia
    Zhou, F.; Gomi, M.; Fujimoto, M.; Hayase, M.; Marumo, T.; Masutani, H.; Yodoi, J.; Hashimoto, N.; Nozaki, K.; Takagi, Y.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off