Intra-amygdala injection of the substance P (NK 1 receptor) antagonist L-760735 inhibits neonatal vocalisations in guinea-pigs

Intra-amygdala injection of the substance P (NK 1 receptor) antagonist L-760735 inhibits neonatal... The involvement of the basolateral amygdala in mediating the inhibition of neonatal vocalisation by substance P (NK 1 receptor) antagonists was examined. These studies determined whether the time course for separation-induced vocalisations in guinea-pig pups coincided with NK 1 receptor internalisation (a marker of substance P release) in the amygdala, and whether vocalisations could be blocked by focal injection of the NK 1 receptor antagonist L-760735 into this brain region. The peak period for neonatal vocalisations occurred 5–10 min following maternal separation. This coincided with the peak increase in the number of cells in the basolateral amygdala exhibiting NK 1 receptor endocytosis, consistent with the proposal that substance P is released in the amygdala as a result of isolation stress. Focal injection of L-760735 (15 nmol per side) but not L-770765 (an analogue of L-760735 which has low NK 1 receptor affinity) into the basolateral amygdala attenuated separation-induced vocalisations. In contrast, injection of L-760735 (15 nmol per side) into the dorsal ventricular nucleus of the thalamus, a region with relatively low density of NK 1 receptors, had no effect on neonatal vocalisations. These findings are consistent with other evidence that the amygdala is one possible site of action for the inhibition of neonatal vocalisations by substance P antagonists. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuropharmacology Elsevier

Intra-amygdala injection of the substance P (NK 1 receptor) antagonist L-760735 inhibits neonatal vocalisations in guinea-pigs

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Publisher
Elsevier
Copyright
Copyright © 2001 Elsevier Science Ltd
ISSN
0028-3908
eISSN
1873-7064
DOI
10.1016/S0028-3908(01)00051-X
Publisher site
See Article on Publisher Site

Abstract

The involvement of the basolateral amygdala in mediating the inhibition of neonatal vocalisation by substance P (NK 1 receptor) antagonists was examined. These studies determined whether the time course for separation-induced vocalisations in guinea-pig pups coincided with NK 1 receptor internalisation (a marker of substance P release) in the amygdala, and whether vocalisations could be blocked by focal injection of the NK 1 receptor antagonist L-760735 into this brain region. The peak period for neonatal vocalisations occurred 5–10 min following maternal separation. This coincided with the peak increase in the number of cells in the basolateral amygdala exhibiting NK 1 receptor endocytosis, consistent with the proposal that substance P is released in the amygdala as a result of isolation stress. Focal injection of L-760735 (15 nmol per side) but not L-770765 (an analogue of L-760735 which has low NK 1 receptor affinity) into the basolateral amygdala attenuated separation-induced vocalisations. In contrast, injection of L-760735 (15 nmol per side) into the dorsal ventricular nucleus of the thalamus, a region with relatively low density of NK 1 receptors, had no effect on neonatal vocalisations. These findings are consistent with other evidence that the amygdala is one possible site of action for the inhibition of neonatal vocalisations by substance P antagonists.

Journal

NeuropharmacologyElsevier

Published: Jul 1, 2001

References

  • Investigation into species variants in tachykinin NK 1 receptors by use of the non-peptide antagonist, CP-96,345
    Beresford, I.J.M; Birch, P.J; Hagan, R.M; Ireland, S.J
  • Neonatal ablations of the amygdala and inferior temporal cortex alter the vocal response to social separation in rhesus macaques
    Newman, J.D; Bachevalier, J
  • Pharmacological blockade or genetic deletion of substance P (NK 1 ) receptors attenuates neonatal vocalisation in guinea pigs and mice
    Rupniak, N.M.J; Carlson, E.C; Harrison, T; Oates, B; Seward, E; Owen, S
  • Effects of benzodiazepine and GABA antagonists on anticonflict effects of antianxiety drugs injected into the rat amygdala in a water-lick suppression test
    Shibata, S; Yamashita, K; Yamamoto, E; Ozaki, T; Ueki, S
  • Reduction of substance P after chronic antidepressant treatment in the striatum, substantia nigra and amygdala of the rat
    Shirayama, Y; Mitsushio, H; Takashima, M; Ichikawa, H; Takahishi, K

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