Inhibition of Peroxynitrite-Mediated Tyrosine Nitration by Catechin Polyphenols

Inhibition of Peroxynitrite-Mediated Tyrosine Nitration by Catechin Polyphenols Peroxynitrite is a cytotoxic species generated by the reaction between superoxide and nitric oxide. The ability of catechins and their gallate esters to decrease peroxynitrite-induced nitration of tyrosine and to limit surface charge alteration of low density lipoprotein (LDL) was investigated. All compounds tested were found to be potent peroxynitrite scavengers preventing the nitration of tyrosine. The ability of the catechin polyphenols at 10 μM to minimise tyrosine nitration induced by peroxynitrite (500 μM) was ECG (38.1 ± 3.6%) ≈ EGCG (32.1 ± 7.5%) ≈ gallic acid (32.1 ± 1.9%) > catechin (23.9 ± 5.4%) ≈ epicatechin (22.9 ± 3.3%) ≈ EGC (19.9 ± 2.0%). Trolox (10 μM) was used as the standard for comparative purposes and was found to be less effective than the polyphenols in inhibiting tyrosine nitration (13.6 ± 2.9%). The catechin polyphenols were also found to offer protection from peroxynitrite-induced modification of critical amino acids of apolipoprotein B-100 of LDL which contribute towards its surface charge. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

Inhibition of Peroxynitrite-Mediated Tyrosine Nitration by Catechin Polyphenols

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Publisher
Elsevier
Copyright
Copyright © 1997 Academic Press
ISSN
0006-291x
DOI
10.1006/bbrc.1997.6254
Publisher site
See Article on Publisher Site

Abstract

Peroxynitrite is a cytotoxic species generated by the reaction between superoxide and nitric oxide. The ability of catechins and their gallate esters to decrease peroxynitrite-induced nitration of tyrosine and to limit surface charge alteration of low density lipoprotein (LDL) was investigated. All compounds tested were found to be potent peroxynitrite scavengers preventing the nitration of tyrosine. The ability of the catechin polyphenols at 10 μM to minimise tyrosine nitration induced by peroxynitrite (500 μM) was ECG (38.1 ± 3.6%) ≈ EGCG (32.1 ± 7.5%) ≈ gallic acid (32.1 ± 1.9%) > catechin (23.9 ± 5.4%) ≈ epicatechin (22.9 ± 3.3%) ≈ EGC (19.9 ± 2.0%). Trolox (10 μM) was used as the standard for comparative purposes and was found to be less effective than the polyphenols in inhibiting tyrosine nitration (13.6 ± 2.9%). The catechin polyphenols were also found to offer protection from peroxynitrite-induced modification of critical amino acids of apolipoprotein B-100 of LDL which contribute towards its surface charge.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Mar 6, 1997

References

  • Arch. Biochem. Biophys.
    Radi, R.; Beckman, J.S.; Bush, K.M.; Freeman, B.A.

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