Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and Atlantic salmon

Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and... Genetic variation and inheritance of random amplified polymorphic DNA (RAPD) markers were studied in 20 intra- and interspecific crosses between nonanadromous salmon ( Salmo salar ) and brown trout ( S. trutta ). Altogether 21 parent fish and 166 F 1 individuals were studied. Three of the 40 primers tested produced screenable and reproducible DNA markers which separated nonanadromous salmon and brown trout. The inheritance of RAPD markers was strictly Mendelian and dominant in all types of crosses. Nonparental bands were not generated from DNAs of F 1 individuals and it is suggested that the frequency of nonparental bands should be less than 0.03% of all bands. The amount of genetic variation was generally low since nonanadromous salmon were totally monomorphic and in brown trout three primers produced six polymorphic DNA fragments. Mean similarity ( s ̄ ) among 11 unrelated specimens of brown trout was 0.941 and between species ( s ̄ ) was 0.338. Among parents, 11 out of 27 and 14 out of 24 DNA fragments were unique for nonanadromous salmon and brown trout, respectively. It is concluded that the RAPD method is one of the most time-saving and the least expensive noninvasive method for detection of interspecific hybridization. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Aquaculture Elsevier

Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and Atlantic salmon

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Publisher
Elsevier
Copyright
Copyright © 1997 Elsevier Ltd
ISSN
0044-8486
D.O.I.
10.1016/S0044-8486(96)01529-3
Publisher site
See Article on Publisher Site

Abstract

Genetic variation and inheritance of random amplified polymorphic DNA (RAPD) markers were studied in 20 intra- and interspecific crosses between nonanadromous salmon ( Salmo salar ) and brown trout ( S. trutta ). Altogether 21 parent fish and 166 F 1 individuals were studied. Three of the 40 primers tested produced screenable and reproducible DNA markers which separated nonanadromous salmon and brown trout. The inheritance of RAPD markers was strictly Mendelian and dominant in all types of crosses. Nonparental bands were not generated from DNAs of F 1 individuals and it is suggested that the frequency of nonparental bands should be less than 0.03% of all bands. The amount of genetic variation was generally low since nonanadromous salmon were totally monomorphic and in brown trout three primers produced six polymorphic DNA fragments. Mean similarity ( s ̄ ) among 11 unrelated specimens of brown trout was 0.941 and between species ( s ̄ ) was 0.338. Among parents, 11 out of 27 and 14 out of 24 DNA fragments were unique for nonanadromous salmon and brown trout, respectively. It is concluded that the RAPD method is one of the most time-saving and the least expensive noninvasive method for detection of interspecific hybridization.

Journal

AquacultureElsevier

Published: Jun 1, 1997

References

  • Hybridization and introgression among species of sunfish ( Lepomis ): analysis by mitochondrial DNA and allozyme markers
    Avise, J.C.; Saunders, N.C.
  • Applications of random amplified polymorphic DNA (RAPD) in molecular ecology
    Hadrys, H.; Balick, M.; Schierwater, B.
  • Patterns of inheritance with RAPD molecular markers reveal novel types of polymorphism in the honey bee
    Hunt, G.J.; Page, R.E.
  • A simplified protocol for routine total DNA isolation from salmonid fishes
    Taggart, J.B.; Hynes, R.A.; Prodöhl, P.A.; Ferguson, A.

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