Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging

Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging Multiphoton microscopy (MPM) is the method of choice for investigating cells and cellular functions in deep tissue sections and organs. Here we present the setup and applications of infrared-(IR-)MPM using excitation wavelengths above 1080 nm. IR-MPM enables the use of red fluorophores and fluorescent proteins, doubles imaging depth, improves second harmonic generation of tissue structures, and strongly reduces phototoxicity and photobleaching, compared with conventional MPM. Furthermore, it still provides subcellular resolution at depths of several hundred micrometers and thus will enhance long-term live cell and deep tissue microscopy. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Opinion in Biotechnology Elsevier

Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging

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Publisher
Elsevier
Copyright
Copyright © 2009 Elsevier Ltd
ISSN
0958-1669
D.O.I.
10.1016/j.copbio.2009.02.008
Publisher site
See Article on Publisher Site

Abstract

Multiphoton microscopy (MPM) is the method of choice for investigating cells and cellular functions in deep tissue sections and organs. Here we present the setup and applications of infrared-(IR-)MPM using excitation wavelengths above 1080 nm. IR-MPM enables the use of red fluorophores and fluorescent proteins, doubles imaging depth, improves second harmonic generation of tissue structures, and strongly reduces phototoxicity and photobleaching, compared with conventional MPM. Furthermore, it still provides subcellular resolution at depths of several hundred micrometers and thus will enhance long-term live cell and deep tissue microscopy.

Journal

Current Opinion in BiotechnologyElsevier

Published: Feb 1, 2009

References

  • Two-photon laser scanning fluorescence microscopy
    Denk, W.; Strickler, J.H.; Webb, W.W.
  • Multiphoton excitation spectra in biological samples
    Dickinson, M.E.; Simbuerger, E.; Zimmermann, B.; Waters, C.W.; Fraser, S.E.
  • Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy
    Eggeling, C.; Volkmer, A.; Seidel, C.A.
  • Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model
    Alexander, S.; Koehl, G.E.; Hirschberg, M.; Geissler, E.K.; Friedl, P.
  • High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution
    Koenig, K.; Riemann, I.

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