Influence of Oligomer Chain Length on the Antioxidant Activity of Procyanidins

Influence of Oligomer Chain Length on the Antioxidant Activity of Procyanidins The antioxidant activity of catechin monomers and procyanidin (dimers to hexamers) fractions purified from cocoa was studied in two in vitro systems: liposomes and human LDL. Liposome oxidation (evaluated as formation of 2-thiobarbituric acid reactive substances) was initiated with 2,2′-azobis (2-amidinopropane) hydrochloride (AAPH), 2,2′-azobis (2,4-dimethylvaleronitrile) (AMVN), iron/ascorbate, or UV-C; LDL oxidation (evaluated as formation of conjugated dienes) was initiated with Cu 2+ or AAPH. Catechin monomers and procyanidin fractions inhibited both liposome and LDL oxidation. Monomers, dimers, and trimers fractions were the most effective antioxidants when liposome oxidation was initiated in the aqueous phase. When oxidation was initiated in the lipid domains, higher molecular weight procyanidins were the most effective. All fractions significantly inhibited Cu-mediated LDL oxidation; no significant effect of procyanidin molecular weight was observed. The hexamer fraction was the least effective with respect to preventing AAPH initiated LDL oxidation. Results reported herein give further evidence on the influence of the oligomer chain length on the antioxidant protection by procyanidins. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

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Publisher
Elsevier
Copyright
Copyright © 2000 Academic Press
ISSN
0006-291x
DOI
10.1006/bbrc.2000.3571
Publisher site
See Article on Publisher Site

Abstract

The antioxidant activity of catechin monomers and procyanidin (dimers to hexamers) fractions purified from cocoa was studied in two in vitro systems: liposomes and human LDL. Liposome oxidation (evaluated as formation of 2-thiobarbituric acid reactive substances) was initiated with 2,2′-azobis (2-amidinopropane) hydrochloride (AAPH), 2,2′-azobis (2,4-dimethylvaleronitrile) (AMVN), iron/ascorbate, or UV-C; LDL oxidation (evaluated as formation of conjugated dienes) was initiated with Cu 2+ or AAPH. Catechin monomers and procyanidin fractions inhibited both liposome and LDL oxidation. Monomers, dimers, and trimers fractions were the most effective antioxidants when liposome oxidation was initiated in the aqueous phase. When oxidation was initiated in the lipid domains, higher molecular weight procyanidins were the most effective. All fractions significantly inhibited Cu-mediated LDL oxidation; no significant effect of procyanidin molecular weight was observed. The hexamer fraction was the least effective with respect to preventing AAPH initiated LDL oxidation. Results reported herein give further evidence on the influence of the oligomer chain length on the antioxidant protection by procyanidins.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Oct 5, 2000

References

  • Polyphenols in chocolate, which have antioxidant activity, modulate immune functions in humans in vitro
    Sanbongi, C.; Suzuki, N.; Sakane, T.
  • Sc 3+ , Ga 3+ , In 3+ , Y 3+ , and Be 2+ promote changes in membrane physical properties and facilitate Fe 2+ -initiated lipid peroxidation
    Verstraeten, S.V.; Oteiza, P.I.
  • Sequential ultracentrifugation micromethod for separation of serum lipoproteins and assays of lipids, apolipoproteins, and lipoprotein particles
    Brousseau, T.; Clavey, V.; Bard, J.M.; Fruchart, J.C.
  • Enzymatic determination of total serum cholesterol
    Allain, C.C.; Poon, L.S.; Chan, C.S.; Richmonds, W.; Fu, P.C.
  • Decomposition of cocoa procyanidins in the gastric milieu
    Spencer, J.P.E.; Chaudry, F.; Pannala, A.S.; Srai, S.K.; Debnam, E.; Rice-Evans, C.

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