Increased serotonin release in mice frontal cortex and hippocampus induced by acute physiological stressors

Increased serotonin release in mice frontal cortex and hippocampus induced by acute physiological... The effects of acute physiological stressors (5 s tail pinch, handling and forced swimming at +25 and +5 °C for 3 min each) on serotonin (5-HT) release in the mouse brain were investigated using in vivo microdialysis. The extracellular 5-HT levels were determined by a newly developed highly-sensitive and selective high-performance liquid chromatography method based on derivatization with benzylamine and fluorescence detection. The basal levels of 5-HT in 3 min microdialysates from the ventral hippocampus and frontal cortex were 0.68±0.21 and 0.75±0.28 fmol/6 μl ( n =24), respectively. All three stressors caused an immediate, significant and reversible increase (handling: 150%; swimming: 240%) of extracellular 5-HT levels in both brain structures, suggesting a more dynamic role played by the serotonergic system in response to acute stress. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuroscience Letters Elsevier

Increased serotonin release in mice frontal cortex and hippocampus induced by acute physiological stressors

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Ltd
ISSN
0304-3940
DOI
10.1016/S0304-3940(02)00029-0
Publisher site
See Article on Publisher Site

Abstract

The effects of acute physiological stressors (5 s tail pinch, handling and forced swimming at +25 and +5 °C for 3 min each) on serotonin (5-HT) release in the mouse brain were investigated using in vivo microdialysis. The extracellular 5-HT levels were determined by a newly developed highly-sensitive and selective high-performance liquid chromatography method based on derivatization with benzylamine and fluorescence detection. The basal levels of 5-HT in 3 min microdialysates from the ventral hippocampus and frontal cortex were 0.68±0.21 and 0.75±0.28 fmol/6 μl ( n =24), respectively. All three stressors caused an immediate, significant and reversible increase (handling: 150%; swimming: 240%) of extracellular 5-HT levels in both brain structures, suggesting a more dynamic role played by the serotonergic system in response to acute stress.

Journal

Neuroscience LettersElsevier

Published: Mar 1, 2002

References

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