In vivo and in organello assessment of OXPHOS activities

In vivo and in organello assessment of OXPHOS activities Mitochondrial OXPHOS defects are responsible for a large group of human diseases and have been associated with degenerative disorders and aging. The accurate in vivo and in organello biochemical assessment of the OXPHOS system is necessary for the diagnosis and investigation of such conditions. Here I describe a set of accurate polarographic and spectrophotometric assays that use relatively small amounts of biological material (cells or isolated mitochondria) and discuss the biochemical parameters appropriate for discriminating partial OXPHOS defects. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Methods Elsevier

In vivo and in organello assessment of OXPHOS activities

Methods, Volume 26 (4) – Apr 2, 2002

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Science (USA)
ISSN
1046-2023
eISSN
1095-9130
DOI
10.1016/S1046-2023(02)00036-1
Publisher site
See Article on Publisher Site

Abstract

Mitochondrial OXPHOS defects are responsible for a large group of human diseases and have been associated with degenerative disorders and aging. The accurate in vivo and in organello biochemical assessment of the OXPHOS system is necessary for the diagnosis and investigation of such conditions. Here I describe a set of accurate polarographic and spectrophotometric assays that use relatively small amounts of biological material (cells or isolated mitochondria) and discuss the biochemical parameters appropriate for discriminating partial OXPHOS defects.

Journal

MethodsElsevier

Published: Apr 2, 2002

References

  • J. Mol. Biol.
    Hofhaus, G.; Weiss, H.; Leonard, K.

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