Improved fish species identification by use of lab-on-a-chip technology

Improved fish species identification by use of lab-on-a-chip technology Work reported here shows the outcome of improvements made to a published PCR-RFLP approach for fish species identification. The objective of the improved method was to replace the gel-electrophoretic steps for fragment separation, detection and analysis, by employing a chip-based capillary electrophoresis (CE) system. Fragment resolution on the system was sensitive, with detection of small fragments not observed with the published conventional gel-based method. Experimental repeatability was less than 3%, allowing species identification without the need to run reference materials with every sample. Using DNA admixtures, the discrimination of 5% salmon DNA in trout DNA was readily achieved. Results showed that the CE system was quick and easy to use, providing post-restriction digestion results for 12 samples within 40 min. This, along with the relatively low cost of the instrument, should make this method suitable for use in a wide range of analytical laboratories involved with species identification. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food Control Elsevier

Improved fish species identification by use of lab-on-a-chip technology

Loading next page...
 
/lp/elsevier/improved-fish-species-identification-by-use-of-lab-on-a-chip-0fgSdpQThr
Publisher site
See Article on Publisher Site

Abstract

Work reported here shows the outcome of improvements made to a published PCR-RFLP approach for fish species identification. The objective of the improved method was to replace the gel-electrophoretic steps for fragment separation, detection and analysis, by employing a chip-based capillary electrophoresis (CE) system. Fragment resolution on the system was sensitive, with detection of small fragments not observed with the published conventional gel-based method. Experimental repeatability was less than 3%, allowing species identification without the need to run reference materials with every sample. Using DNA admixtures, the discrimination of 5% salmon DNA in trout DNA was readily achieved. Results showed that the CE system was quick and easy to use, providing post-restriction digestion results for 12 samples within 40 min. This, along with the relatively low cost of the instrument, should make this method suitable for use in a wide range of analytical laboratories involved with species identification.

Journal

Food ControlElsevier

Published: Sep 1, 2005

References

  • Identification of flatfish species using polymerase chain reaction (PCR) amplification and restriction analysis of the cytochrome b gene
    Cespedes, A.; Garcia, T.; Carrera, E.; Gonzalez, I.; Sanz, B.; Hernandez, P.E.; Martin, R.
  • Identification of fish species using random amplified polymorphic DNA (RAPD)
    Partis, L.; Wells, R.J.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off