Immunocytochemical localization of polyamines in the tiger salamander retina

Immunocytochemical localization of polyamines in the tiger salamander retina The polyamines spermine and spermidine are present in neural tissue, but their functions there are not well understood. Recent work suggests that the NMDA subtype of glutamate receptors, other glutamate receptor subtypes, and certain K + -channels, are neural targets for polyamines. To better understand the neuron-specific roles of polyamines, we have developed antibodies that interact with spermine and spermidine in aldehyde-fixed tissue and used these antibodies in immunocytochemical studies to determine the cellular localization of these polyamines in the tiger salamander retina. The affinity-purified, polyclonal antibodies were highly specific for spermine and spermidine, exhibiting < 1 % cross reactivity with putrescine, and virtually no cross-reactivity with GABA, arginine, lysine, or glutaraldehyde. Polyamine labeling was most abundant in cells in the inner half of the inner nuclear layer and in the ganglion cell layer. Some cells in the outer half of the inner nuclear layer are labeled, and there was some labeling in both synaptic layers. Double-labeling experiments indicated (1) all GABAergic amacrine cells were polyamine-positive; and (2) all ganglion cells (identified by back-filling after microinjections of rhodamine in the optic nerve) were polyamine-positive. These results are consistent with a role for polyamines as modulators of NMDA receptor function and channel function in the inner retina. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Immunocytochemical localization of polyamines in the tiger salamander retina

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Ltd
ISSN
0006-8993
D.O.I.
10.1016/0006-8993(95)01558-2
Publisher site
See Article on Publisher Site

Abstract

The polyamines spermine and spermidine are present in neural tissue, but their functions there are not well understood. Recent work suggests that the NMDA subtype of glutamate receptors, other glutamate receptor subtypes, and certain K + -channels, are neural targets for polyamines. To better understand the neuron-specific roles of polyamines, we have developed antibodies that interact with spermine and spermidine in aldehyde-fixed tissue and used these antibodies in immunocytochemical studies to determine the cellular localization of these polyamines in the tiger salamander retina. The affinity-purified, polyclonal antibodies were highly specific for spermine and spermidine, exhibiting < 1 % cross reactivity with putrescine, and virtually no cross-reactivity with GABA, arginine, lysine, or glutaraldehyde. Polyamine labeling was most abundant in cells in the inner half of the inner nuclear layer and in the ganglion cell layer. Some cells in the outer half of the inner nuclear layer are labeled, and there was some labeling in both synaptic layers. Double-labeling experiments indicated (1) all GABAergic amacrine cells were polyamine-positive; and (2) all ganglion cells (identified by back-filling after microinjections of rhodamine in the optic nerve) were polyamine-positive. These results are consistent with a role for polyamines as modulators of NMDA receptor function and channel function in the inner retina.

Journal

Brain ResearchElsevier

Published: Mar 25, 1996

References

  • Multiple effects of spermine on N -methyl- d -aspartic acid receptor responses of rat cultured hippocampal neurones
    Benveniste, M.; Mayer, M.L.
  • Polyamine localization and biosynthesis in chemically fractionated rat retina
    Ientile, R.; Russo, P.; Macaione, S.
  • Synaptic transmission at N -methyl- d -aspartate receptors in the proximal retina of the mudpuppy
    Lukasiewicz, P.; McReynolds, J.S.
  • Concomitant activation of two types of glutamate receptor mediates excitation of salamander retinal ganglion cells
    Mittman, S.; Taylor, W.R.; Copenhagen, D.R.
  • Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives
    Smith, M.A.; Davies, P.J.

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