Imaging flow cytometry to assess chromosomal abnormalities in chronic lymphocytic leukaemia

Imaging flow cytometry to assess chromosomal abnormalities in chronic lymphocytic leukaemia Methods 134–135 (2018) 32–40 Contents lists available at ScienceDirect Methods journal homepage: www.elsevier.com/locate/ymeth Imaging flow cytometry to assess chromosomal abnormalities in chronic lymphocytic leukaemia a,1 a,b,1 a,c a,d a,d a,b,d Henry Hui , Kathryn A. Fuller , Hun Chuah , James Liang , Hasib Sidiqi , Dejan Radeski , a,b,⇑ Wendy N. Erber Translational Cancer Pathology Laboratory, School of Biomedical Sciences, The University of Western Australia, Crawley, Australia PathWest Laboratory Medicine, Nedlands, Australia Department of Haematology, Royal Perth Hospital, Perth, Australia Department of Haematology, Sir Charles Gairdner Hospital, Nedlands, Australia ar ti c l e i nf o ab stra ct Article history: Chronic Lymphocytic Leukaemia (CLL), the most common leukaemia in the Western world, has a charac- Received 1 September 2017 teristic phenotype and prognosis largely defined by the presence of cytogenetic aberrations. The gold Received in revised form 8 November 2017 standard for detecting these cytogenetic abnormalities is interphase fluorescence in situ hybridisation Accepted 13 November 2017 (FISH) performed on cell smears or tissue sections on glass slides. Fluorescently labelled DNA probes bind Available online 15 November 2017 to specific chromosomal regions and the signal detected by fluorescent microscopy. Generally only 200 cells are assessed and the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Methods Elsevier

Imaging flow cytometry to assess chromosomal abnormalities in chronic lymphocytic leukaemia

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Inc.
ISSN
1046-2023
eISSN
1095-9130
D.O.I.
10.1016/j.ymeth.2017.11.003
Publisher site
See Article on Publisher Site

Abstract

Methods 134–135 (2018) 32–40 Contents lists available at ScienceDirect Methods journal homepage: www.elsevier.com/locate/ymeth Imaging flow cytometry to assess chromosomal abnormalities in chronic lymphocytic leukaemia a,1 a,b,1 a,c a,d a,d a,b,d Henry Hui , Kathryn A. Fuller , Hun Chuah , James Liang , Hasib Sidiqi , Dejan Radeski , a,b,⇑ Wendy N. Erber Translational Cancer Pathology Laboratory, School of Biomedical Sciences, The University of Western Australia, Crawley, Australia PathWest Laboratory Medicine, Nedlands, Australia Department of Haematology, Royal Perth Hospital, Perth, Australia Department of Haematology, Sir Charles Gairdner Hospital, Nedlands, Australia ar ti c l e i nf o ab stra ct Article history: Chronic Lymphocytic Leukaemia (CLL), the most common leukaemia in the Western world, has a charac- Received 1 September 2017 teristic phenotype and prognosis largely defined by the presence of cytogenetic aberrations. The gold Received in revised form 8 November 2017 standard for detecting these cytogenetic abnormalities is interphase fluorescence in situ hybridisation Accepted 13 November 2017 (FISH) performed on cell smears or tissue sections on glass slides. Fluorescently labelled DNA probes bind Available online 15 November 2017 to specific chromosomal regions and the signal detected by fluorescent microscopy. Generally only 200 cells are assessed and the

Journal

MethodsElsevier

Published: Feb 1, 2018

References

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