Bioresource Technology 249 (2018) 720–728 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech Identiﬁcation of D-carbamoylase for biocatalytic cascade synthesis of D- tryptophan featuring high enantioselectivity Yafei Liu, Guochao Xu, Ruizhi Han, Jinjun Dong, Ye Ni The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: In this study, an enantioselective D-carbamoylase (AcHyuC) was identiﬁed from Arthrobacter crystallopoietes with D-Carbamoylase optimum pH of 8.5, much more compatible with hydantoinase process than other reported D-N-carbamoylases. D-Tryptophan AcHyuC has a substrate preference for aromatic carbamoyl-compounds. The dynamic kinetic resolution (DKR) DKR cascade cascade was developed by combining this AcHyuC with hydantoin racemase from Arthrobacter aurescens Hydantoinase process (AaHyuA) and D-hydantoinase from Agrobacterium tumefaciens (AtHyuH) for enantioselective resolution of L- Arthrobacter crystallopoietes indolylmethylhydantoin into D-Trp. The optimum pH of DKR cascade reaction was determined to be 8.0, and PEG 400 could facilitate the reaction. As much as 80 mM L-indolylmethylhydantoin could be fully converted to D- −1 −1 Trp within 12 h at 0.5 L scale, with 99.4% yield, > 99.9% e.e. and productivity of 36.6 g L d . This study provides
Bioresource Technology – Elsevier
Published: Feb 1, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera