Journal of Food Composition and Analysis 71 (2018) 8–16 Contents lists available at ScienceDirect Journal of Food Composition and Analysis journal homepage: www.elsevier.com/locate/jfca Original research article Identiﬁcation of chickpea (Cicer arietinum) in foods using a novel real-time polymerase chain reaction detection method a, a a a a Kosuke Nakamura , Takumi Ishigaki , Tomoko Kobayashi , Shinya Kimata , Keisuke Soga , a,b c c c c Uki Fujii , Masahiro Kishine , Reona Takabatake , Junichi Mano , Kazumi Kitta , b a a Hiroshi Kawakami , Tomoko Nishimaki-Mogami , Kazunari Kondo National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan Kyoritsu Women's University, 2-2-1 Hitotsubashi, Chiyoda-ku, Tokyo 101-8437, Japan Food Research Institute, NARO, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan ARTIC L E I NF O ABSTRAC T Keywords: A novel real-time polymerase chain reaction (PCR) method for detecting chickpea was developed. From Food analysis homologous sequences of 9-cis-epoxycarotenoid dioxygenase gene (NCED) among leguminous species, chickpea’s Food composition NCED was cloned, and the Southern-blot analysis showed that NCED is a single copy gene in a haploid genome. Cicer arietinum Its coding sequences at the 5′ terminus were found unique to chickpea and conserved among various
Journal of Food Composition and Analysis – Elsevier
Published: Aug 1, 2018
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