Identification of a Unique Monocarboxylate Transporter (MCT3) in Retinal Pigment Epithelium

Identification of a Unique Monocarboxylate Transporter (MCT3) in Retinal Pigment Epithelium The retinal pigment epithelium transports lactate between two tissue compartments, the interphotoreceptor matrix and the choriocapillaris. In this report we describe a 2.45-kb cDNA isolated from a chick cDNA RPE library that encodes a membrane protein found only in RPE cells. The deduced protein has 542 amino acids with twelve putative membrane spanning domains. The cDNA has been designated MCT3 based on its 45% identity in amino acid sequence and structural similarity with the monocarboxylate transporters MCT1 and MCT2. Stable transfectants ( p Cl-neo/MCT3), made in a rat thyroid epithelial cell line (FRTL-5), express MCT3 RNA. Transfectants had enhanced pyruvate uptake (used as a measure of lactate uptake) which was proton-dependent and inhibited by α-cyano-4-hydroxycinnamate. In summary, MCT3's unique expression in RPE cells, multiple potential phosphorylation sites, and basolateral distribution suggest that MCT3 may regulate lactate levels in the interphotoreceptor space. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

Identification of a Unique Monocarboxylate Transporter (MCT3) in Retinal Pigment Epithelium

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Publisher
Elsevier
Copyright
Copyright © 1997 Academic Press
ISSN
0006-291x
D.O.I.
10.1006/bbrc.1997.6588
Publisher site
See Article on Publisher Site

Abstract

The retinal pigment epithelium transports lactate between two tissue compartments, the interphotoreceptor matrix and the choriocapillaris. In this report we describe a 2.45-kb cDNA isolated from a chick cDNA RPE library that encodes a membrane protein found only in RPE cells. The deduced protein has 542 amino acids with twelve putative membrane spanning domains. The cDNA has been designated MCT3 based on its 45% identity in amino acid sequence and structural similarity with the monocarboxylate transporters MCT1 and MCT2. Stable transfectants ( p Cl-neo/MCT3), made in a rat thyroid epithelial cell line (FRTL-5), express MCT3 RNA. Transfectants had enhanced pyruvate uptake (used as a measure of lactate uptake) which was proton-dependent and inhibited by α-cyano-4-hydroxycinnamate. In summary, MCT3's unique expression in RPE cells, multiple potential phosphorylation sites, and basolateral distribution suggest that MCT3 may regulate lactate levels in the interphotoreceptor space.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: May 8, 1997

References

  • Exp. Cell Res.
    Philp, N.J.; Chu, P.; Pan, T.C.; Chu, M.L.; Stark, K.; Boettiger, D.; Yoon, H.; Kieber-Emmons, T.

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