Identification of a poly(3-hydroxybutyrate)-degrading bacterium isolated from coastal seawater in Japan as Shewanella sp.

Identification of a poly(3-hydroxybutyrate)-degrading bacterium isolated from coastal seawater in... A poly[(R)-3-hydroxybutyrate] [P(3HB)]-degrading bacterium (strain JKCM-AJ-6,1α) was isolated from coastal seawater near the port of Yaizu, Suruga Bay, Japan. The strain grew well in a marine mineral medium supplemented with P(3HB) powder as the sole carbon source. Phylogenetic analysis revealed that the strain JKCM-AJ-6,1α belonged to the genus Shewanella. The isolate was unable to grow at temperatures above 50 °C, and the optimum range of growth temperatures was 30 °C–37 °C; however, the optimum temperature for degradation of P(3HB) was 15 °C. The maximum specific growth rate (μ = 1.0 h−1) was attained at a NaCl concentration of 0.2 M, but the strain was able to grow even in the presence of 0.8 M NaCl. This suggests that the strain is a typical marine bacterium. This is the first report showing P(3HB) depolymerase activity in a member of the genus Shewanella. It is concluded that the genus Shewanella may be one of the key bacterial groups involved in the P(3HB) cycle in marine environments. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Polymer Degradation and Stability Elsevier

Identification of a poly(3-hydroxybutyrate)-degrading bacterium isolated from coastal seawater in Japan as Shewanella sp.

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Publisher
Elsevier
Copyright
Copyright © 2016 Elsevier Ltd
ISSN
0141-3910
D.O.I.
10.1016/j.polymdegradstab.2016.05.008
Publisher site
See Article on Publisher Site

Abstract

A poly[(R)-3-hydroxybutyrate] [P(3HB)]-degrading bacterium (strain JKCM-AJ-6,1α) was isolated from coastal seawater near the port of Yaizu, Suruga Bay, Japan. The strain grew well in a marine mineral medium supplemented with P(3HB) powder as the sole carbon source. Phylogenetic analysis revealed that the strain JKCM-AJ-6,1α belonged to the genus Shewanella. The isolate was unable to grow at temperatures above 50 °C, and the optimum range of growth temperatures was 30 °C–37 °C; however, the optimum temperature for degradation of P(3HB) was 15 °C. The maximum specific growth rate (μ = 1.0 h−1) was attained at a NaCl concentration of 0.2 M, but the strain was able to grow even in the presence of 0.8 M NaCl. This suggests that the strain is a typical marine bacterium. This is the first report showing P(3HB) depolymerase activity in a member of the genus Shewanella. It is concluded that the genus Shewanella may be one of the key bacterial groups involved in the P(3HB) cycle in marine environments.

Journal

Polymer Degradation and StabilityElsevier

Published: Jul 1, 2016

References

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