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hydrochloride and anti-mouse IgG– horseradish peroxidase conjugates were purchased from Sigma (USA), 4-iodophenol from Aldrich (USA) and 1,10-phenanthroline, Tiron (4,5-dihydroxy-1,3- benzene -disulfonic acid ...
with horseradish peroxidase (HRP) (Abcam ab6656, Cambridge, UK) diluted 10 000-fold in PBST/1% w/v bovine serum albumin for 1 h at 21°C. The liquid was decanted from the wells and the wells washed four times ...
, the membranes were incubated with horseradish peroxidase -conjugated secondary antibodies for 1 h and then washed several times. Protein expression was detected by chemiluminescence using the ECL Western Blotting ...
- horseradish peroxidase (HRP) and ELISA reader. Cell-Based ELISA of Tie-2, CD31, and Flk-1 BMSCs (2 × 104 per well) were seeded in 96-well tissue culture plates coated with 10 μg/ml poly-l-lysine for 30 minutes ...
of peroxidases in the bioluminescence mechanism of this mushroom. As Mn ions stimulate luminescence of N. nambi mycelium, the composition of lignin‐braking enzymic complex of the mushroom can be expected ...
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