Glycoproteomics is an important subdiscipline of proteomics. Mass spectrometry (MS)-based glycoproteomics has relied so far on two levels of analysis, targeting either the released glycans or digested glycopeptides. However, limitations of these approaches, caused by the vast structural microheterogeneity that glycoproteins can exhibit, have become evident. Therefore, concomitant methods enabling deep characterization of all discrete glycoforms are essential to extend our functional understanding of the glycoproteome. Here, we discuss recent progress, particularly in protein-centric approaches. High-resolution native MS appears to be especially promising in revealing the glycoform profile of glycoproteins. We propose that systematically integrating MS data acquired at the glycan, glycopeptide, and glycoprotein levels best enhances our understanding of the glycoproteome.
Trends in Biotechnology – Elsevier
Published: Jul 1, 2017
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.
All for just $49/month
Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.
Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.
It’s easy to organize your research with our built-in tools.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera