Available online at www.sciencedirect.com ScienceDirect Glycan analysis by ion mobility-mass spectrometry and gas-phase spectroscopy 1,2 1,2 Christian Manz and Kevin Pagel Due to the existence of numerous isomers, the in-depth regiochemistry and branching (connectivity), and the analysis of glycans represents a major challenge. Currently, the stereochemistry (conﬁguration) (Figure 1). Furthermore, majority of glycans are analysed using mass spectrometry glycans may exist in multiple conformations and, in case (MS)-based techniques, which can provide information on of a free reducing end, form an equilibrium between regioisomers but usually fail to differentiate stereoisomers. A different conﬁgurations (i.e. a and b) and ring structures promising approach to overcome this limitation is to implement (i.e. pyranose and furanose) . ion mobility spectrometry (IMS) as an additional gas-phase separation dimension. This review highlights recent The combination of these structural categories fre- developments in which IM-MS was used as a tool for quently results in closely related structural isomers, comprehensive glycan analysis or as rapid screening method which are often difﬁcult to distinguish using established for glycan feature analysis. Furthermore, we summarize a analytical tools. Today, a variety of hyphenated techni- series of very recent investigations in which gas-phase ques are applied to elucidate glycan structures,
Current Opinion in Chemical Biology – Elsevier
Published: Feb 1, 2018
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