Genomic Organization of the Mouse Reelin Gene

Genomic Organization of the Mouse Reelin Gene Reelin is the protein defective in reeler mice, an extensively studied model of brain development. The reelin gene (symbol Reln ) codes for a protein of the extracellular matrix that contains eight successive repeats of 350 to 390 amino acids. In this work, we describe the genomic structure of the mouse reelin gene and the 5′-flanking genomic DNA sequences. The reelin gene is composed of 65 exons spread over approximately 450 kb of genomic DNA. We identified different reelin transcripts, formed by alternative splicing of a microexon as well as by use of two different polyadenylation sites. All splice sites conform to the GT-AG rule, except for the splice donor site of intron 30, which is GC instead of GT. A processed pseudogene is present in intron 42. Its nucleotide sequence is 86% identical to the sequence of the rat RDJ1 cDNA, which codes for a DnaJ-like protein of the Hsp40 family. Comparison of 8 intron positions in mouse and human reelin genes reveals a highly conserved genomic structure, suggesting a similar structure of the whole gene in both species. We identified two transcription start sites embedded within a CpG. The promoter region contains putative recognition sites for the transcription factors Sp1 and AP2 but lacks TATA and CAAT boxes. The presence of tandemly repeated regions in the Reelin protein suggests that gene duplication events occurred during evolution. By comparison of the amino acid sequences of the eight repeats and the positions of introns, we suggest a model for the evolution of the repeat coding portion of the reelin gene from a putative ancestral minigene. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genomics Elsevier

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Publisher
Elsevier
Copyright
Copyright © 1997 Academic Press
ISSN
0888-7543
eISSN
1089-8646
DOI
10.1006/geno.1997.4983
Publisher site
See Article on Publisher Site

Abstract

Reelin is the protein defective in reeler mice, an extensively studied model of brain development. The reelin gene (symbol Reln ) codes for a protein of the extracellular matrix that contains eight successive repeats of 350 to 390 amino acids. In this work, we describe the genomic structure of the mouse reelin gene and the 5′-flanking genomic DNA sequences. The reelin gene is composed of 65 exons spread over approximately 450 kb of genomic DNA. We identified different reelin transcripts, formed by alternative splicing of a microexon as well as by use of two different polyadenylation sites. All splice sites conform to the GT-AG rule, except for the splice donor site of intron 30, which is GC instead of GT. A processed pseudogene is present in intron 42. Its nucleotide sequence is 86% identical to the sequence of the rat RDJ1 cDNA, which codes for a DnaJ-like protein of the Hsp40 family. Comparison of 8 intron positions in mouse and human reelin genes reveals a highly conserved genomic structure, suggesting a similar structure of the whole gene in both species. We identified two transcription start sites embedded within a CpG. The promoter region contains putative recognition sites for the transcription factors Sp1 and AP2 but lacks TATA and CAAT boxes. The presence of tandemly repeated regions in the Reelin protein suggests that gene duplication events occurred during evolution. By comparison of the amino acid sequences of the eight repeats and the positions of introns, we suggest a model for the evolution of the repeat coding portion of the reelin gene from a putative ancestral minigene.

Journal

GenomicsElsevier

Published: Dec 1, 1997

References

  • A YAC contig containing the reeler
    Bar, I.; Lambert de Rouvroit, C.; Royaux, I.; Krizman, D.B.; Dernoncourt, C.; Ruelle, D.; Beckers, M.C.; Goffinet, A.M.
  • ten m , a Drosophila
    Baumgartner, S.; Martin, D.; Hagios, C.; Chiquet-Ehrismann, R.
  • Mechanisms of cortical development: A view from mutations in mice
    Caviness, V.S.; Rakic, P.
  • CpG islands and genes
    Cross, S.H.; Bird, A.P.
  • Evolution of the intron–exon structure of eukaryotic genes
    Long, M.; de Souza, S.J.; Gilbert, W.
  • Reln rl-Alb2 , an allele of reeler
    Royaux, I.; Bernier, B.; Montgomery, J.C.; Flaherty, L.; Goffinet, A.M.
  • Reelin mRNA expression during mouse brain development
    Schiffmann, S.N.; Bernier, B.; Goffinet, A.M.
  • Alternative splicing of fibronectin: Three variants, three functions
    Schwarzbauer, J.E.
  • Chromosomal structure of the human TYRP1 and TYRP2 loci and comparison of the tyrosinase-related protein gene family
    Sturm, R.A.; O'Sullivan, B.J.; Box, N.F.; Smith, A.G.; Simt, S.E.; Puttick, E.R.J.; Parsons, P.G.; Dunn, I.S.
  • Dysfunction of the Orleans reeler gene arising from exon skipping due to transposition of a full-length copy of an active L1 sequence into the skipped exon
    Takahara, T.; Ohsumi, T.; Kuromitsu, J.; Shibata, K.; Sasaki, N.; Okazaki, Y.; Shibata, H.; Sato, S.; Yoshiki, A.; Kusakabe, M.; Muramatsu, M.; Ueki, M.; Okuda, K.; Hayashizaki, Y.
  • Characterization of the exon structure of the Menkes disease gene using vectorette PCR
    Tümer, Z.; Vural, B.; Tonnesen, T.; Chelly, J.; Monaco, A.P.; Horn, N.

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