Genome-wide identification of lectin receptor kinases in pear: Functional characterization of the L-type LecRLK gene PbLRK138

Genome-wide identification of lectin receptor kinases in pear: Functional characterization of the... Lectin receptor-like kinases (LecRLKs) are membrane-bound receptors that are believed to be involved in biotic and abiotic stress responses. However, little is known about the LecRLK family in pear. In this study, a total of 172 LecRLK genes were first identified in the entire pear genome. The 172 LecRLKs were divided into three types (111 G-, 59 L- and two C-types) based on their structure and phylogenetic relationships. LecRLKs gene expressions were detected in different pear tissues including roots, stems, leaves, flowers and fruits, and the most of the 11 selected LecRLKs exhibited similar expression patterns. Furthermore, six selected LecRLKs were shown to be involved in salt stress response. Overexpression of PbLRK138, an L-type LecRLK, caused cell death and induced expression of defense-related genes in Nicotiana benthamiana. Two deletion mutants containing lectin or transmembrane and serine/threonine kinase domains did not trigger cell death. In addition, only the mutant with the transmembrane domain was localized to the plasma membrane. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Gene Elsevier

Genome-wide identification of lectin receptor kinases in pear: Functional characterization of the L-type LecRLK gene PbLRK138

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Ltd
ISSN
0378-1119
eISSN
1879-0038
D.O.I.
10.1016/j.gene.2018.03.077
Publisher site
See Article on Publisher Site

Abstract

Lectin receptor-like kinases (LecRLKs) are membrane-bound receptors that are believed to be involved in biotic and abiotic stress responses. However, little is known about the LecRLK family in pear. In this study, a total of 172 LecRLK genes were first identified in the entire pear genome. The 172 LecRLKs were divided into three types (111 G-, 59 L- and two C-types) based on their structure and phylogenetic relationships. LecRLKs gene expressions were detected in different pear tissues including roots, stems, leaves, flowers and fruits, and the most of the 11 selected LecRLKs exhibited similar expression patterns. Furthermore, six selected LecRLKs were shown to be involved in salt stress response. Overexpression of PbLRK138, an L-type LecRLK, caused cell death and induced expression of defense-related genes in Nicotiana benthamiana. Two deletion mutants containing lectin or transmembrane and serine/threonine kinase domains did not trigger cell death. In addition, only the mutant with the transmembrane domain was localized to the plasma membrane.

Journal

GeneElsevier

Published: Jun 30, 2018

References

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