Molecular Phylogenetics and Evolution 121 (2018) 46–51 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Short Communication Genetic diversity within grouper species and a method for interspeciﬁc hybrid identiﬁcation using DNA barcoding and RYR3 marker Meng Qu, Wei Tang, Qiaohong Liu, Dexiang Wang , Shaoxiong Ding State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, Fujian Province, China ARTICLE I NFO ABSTRACT Keywords: Groupers (family Epinephelidae) are an assemblage of coral reef ﬁshes comprising more than 160 species in 16 Groupers genera, many of which are both environmentally and economically valuable. Because of their similar mor- Intraspeciﬁc diversity phology, variable color patterns, and tendency for interspecies hybridization, morphological identiﬁcation of Hybrid identiﬁcation groupers usually leads to taxonomic confusion. To ﬁnd an eﬀective method for identifying diﬀerent grouper RYR3 marker species and hybrids, evaluate genetic diversity and uncover any synonymous or cryptic species, we sampled a DNA barcoding total of 221 specimens representing 57 species in 9 genera in the China Seas. Both mitochondrial (mt) cyto- chrome oxidase subunit I (COI) and NADH dehydrogenase subunit 2 (ND2) were found to be eﬀective barcoding genes. We also developed an
Molecular Phylogenetics and Evolution – Elsevier
Published: Apr 1, 2018
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