Genetic Analysis of Bristle Loss in Hybrids between Drosophila melanogaster and D. simulans Provides Evidence for Divergence of cis -Regulatory Sequences in the achaete–scute Gene Complex

Genetic Analysis of Bristle Loss in Hybrids between Drosophila melanogaster and D. simulans... The two closely related species of Drosophila, D. melanogaster and D. simulans, display an identical bristle pattern on the notum, but hybrids between the two are lacking a variable number of bristles. We show that the loss is temperature-dependent and provide evidence for two periods of temperature sensitivity. A first period of heat sensitivity occurs during larval development and corresponds to the time when the prepattern of expression of genes whose products activate achaete–scute in the proneural clusters preceding bristle precursor formation is established. A second period of cold sensitivity corresponds to the time of emergence of the bristle precursor cells and the maintenance of their neural fate, a process requiring high levels of Achaete–Scute. Expression of achaete–scute at these two critical periods depends on cis -regulatory elements of the achaete–scute complex (AS-C). The differences between males, which have only one copy of the X-linked AS-C from D. simulans, and females, which have copies from both parental species, are compared, together with the effects of crossing in different rearrangements of the D. melanogaster AS-C that delete regulatory and/or coding sequences. We provide evidence that bristle loss in the hybrids may result from a decrease in the level of transcription at the AS-C and argue that interaction between trans -acting factors and cis -regulatory elements within the AS-C has diverged between the two species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Biology Elsevier

Genetic Analysis of Bristle Loss in Hybrids between Drosophila melanogaster and D. simulans Provides Evidence for Divergence of cis -Regulatory Sequences in the achaete–scute Gene Complex

Developmental Biology, Volume 221 (1) – May 1, 2000

Loading next page...
 
/lp/elsevier/genetic-analysis-of-bristle-loss-in-hybrids-between-drosophila-Po1Nk8zmVM
Publisher
Elsevier
Copyright
Copyright © 2000 Academic Press
ISSN
0012-1606
eISSN
1095-564X
D.O.I.
10.1006/dbio.1999.9661
Publisher site
See Article on Publisher Site

Abstract

The two closely related species of Drosophila, D. melanogaster and D. simulans, display an identical bristle pattern on the notum, but hybrids between the two are lacking a variable number of bristles. We show that the loss is temperature-dependent and provide evidence for two periods of temperature sensitivity. A first period of heat sensitivity occurs during larval development and corresponds to the time when the prepattern of expression of genes whose products activate achaete–scute in the proneural clusters preceding bristle precursor formation is established. A second period of cold sensitivity corresponds to the time of emergence of the bristle precursor cells and the maintenance of their neural fate, a process requiring high levels of Achaete–Scute. Expression of achaete–scute at these two critical periods depends on cis -regulatory elements of the achaete–scute complex (AS-C). The differences between males, which have only one copy of the X-linked AS-C from D. simulans, and females, which have copies from both parental species, are compared, together with the effects of crossing in different rearrangements of the D. melanogaster AS-C that delete regulatory and/or coding sequences. We provide evidence that bristle loss in the hybrids may result from a decrease in the level of transcription at the AS-C and argue that interaction between trans -acting factors and cis -regulatory elements within the AS-C has diverged between the two species.

Journal

Developmental BiologyElsevier

Published: May 1, 2000

References

  • Reduced variation in the yellow-achaete-scute region in natural populations of Drosophila melanogaster
    Aguadé, M; Miyashita, N; Langley, C.H
  • Molecular evolution between Drosophila melanogaster and D. simulans: Reduced codon bias, faster rates of amino acid substitution, and larger proteins in D. melanogaster
    Akashi, H
  • Molecular population genetics of the distal portion of the X chromosome in Drosophila: Evidence for genetic hitchhiking of the yellow-achaete region
    Begun, D; Aquadro, C.F
  • The bristles of hybrids between Drosophila melanogaster and Drosophila simulans
    Biddle, R.L
  • A genetic analysis of pannier, a gene necessary for viability of dorsal tissues and bristle positioning in Drosophila
    Heitzler, P; Haenlin, M; Ramain, P; Calleja, M; Simpson, P
  • Genetics of hybrid inviability in Drosophila
    Hutter, P
  • The Lin-12/Notch signalling pathway and its regulation
    Kimble, J; Simpson, P
  • The melanogaster species subgroup
    Lemeunier, F; David, J.R; Tsacas, L; Ashburner, M
  • An analysis of transvection at the yellow locus of Drosophila melanogaster
    Morris, J.R; Chen, J-L; Filandrinos, S.T; Dunn, R.C; Fisk, R; Geyer, P.K; Wu, C-T
  • Loss of notum macrochaetae as an interspecific hybrid anomaly between Drosophila melanogaster and D. simulans
    Takano, T
  • Rate variation of DNA sequence evolution in Drosophila lineages
    Takano, T

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off