Generation of recombinant protein shells of Johnson grass chlorotic stripe mosaic virus in tobacco plants and their use as drug carrier

Generation of recombinant protein shells of Johnson grass chlorotic stripe mosaic virus in... •Leaves of tobacco plants were inoculated with transformed Agrobacterium rhizogenes harboring coat protein (CP) gene of JgCSMV.•Recombinant JgCSMV CP was expressed to a high level in hairy roots developed from inoculated leaves.•Recombinant CP assembled into 30-nm virus-like particles (VLPs) in vivo in the absence of nucleic acid.•The VLPs were purified and manipulated to allow opening and closure of surface pores.•The VLPs were used as nanocontainers for efficient loading of the anti-cancer drug doxorubicin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

Generation of recombinant protein shells of Johnson grass chlorotic stripe mosaic virus in tobacco plants and their use as drug carrier

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2017.07.003
Publisher site
See Article on Publisher Site

Abstract

•Leaves of tobacco plants were inoculated with transformed Agrobacterium rhizogenes harboring coat protein (CP) gene of JgCSMV.•Recombinant JgCSMV CP was expressed to a high level in hairy roots developed from inoculated leaves.•Recombinant CP assembled into 30-nm virus-like particles (VLPs) in vivo in the absence of nucleic acid.•The VLPs were purified and manipulated to allow opening and closure of surface pores.•The VLPs were used as nanocontainers for efficient loading of the anti-cancer drug doxorubicin.

Journal

Journal of Virological MethodsElsevier

Published: Oct 1, 2017

References

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