•Developed a self-excisable HCMV BAC, TB40/E/Cre.•Found genome length-associated differences in growth of TB40/E derivatives.•Utilized the TB40/E/Cre-based system for transduction of exogenous proteins.•Regulated stability of transduced proteins over periods as short as 2h.•TB40/E/Cre has diverse applications for study of HCMV replication and cell biology.
Virus Research – Elsevier
Published: Oct 15, 2017
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