Fundamentals of the psoralen-based Helinx ™ technology for inactivation of infectious pathogens and leukocytes in platelets and plasma

Fundamentals of the psoralen-based Helinx ™ technology for inactivation of infectious pathogens... Psoralens plus ultraviolet A (UVA) light inactivate viruses and bacteria as well as leukocytes. A system employing the synthetic psoralen compound amotosalen hydrochloride (S-59), in combination with UVA light, is being developed to decontaminate platelet concentrates and plasma in a blood-bank setting. S-59 is a heterocyclic psoralen compound that reacts by a three-step process with nucleic acids (NAs): (1) S-59 intercalates into the double helix; (2) upon illumination with long-wavelength ultraviolet light (UVA), it covalently attaches to a single strand, forming a monoadduct; and (3) additional illumination causes a photoreaction of the monoadduct with the second NA strand, resulting in an interstrand crosslink. The reaction occurs with the genomic material of DNA- and RNA-based viruses and occurs in genomes that are single stranded as well as double stranded. Inactivation rate is related to genome size. Large genomes such as those in leukocytes are far more susceptible to inactivation than are viruses such as hepatitis B virus (HBV), which is inactivated (> 10 5 logs) under conditions being developed for blood-bank use. The efficiency of the process is affected by a number of practical considerations such as solution components and light source. The S-59 photochemical treatment process (PCT) has been optimized for platelet concentrates as currently processed for transfusion. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Seminars in Hematology Elsevier

Fundamentals of the psoralen-based Helinx ™ technology for inactivation of infectious pathogens and leukocytes in platelets and plasma

Seminars in Hematology, Volume 38 – Oct 1, 2001

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Publisher
Elsevier
Copyright
Copyright © 2001 Elsevier Ltd
ISSN
0037-1963
eISSN
1532-8686
D.O.I.
10.1016/S0037-1963(01)90118-0
Publisher site
See Article on Publisher Site

Abstract

Psoralens plus ultraviolet A (UVA) light inactivate viruses and bacteria as well as leukocytes. A system employing the synthetic psoralen compound amotosalen hydrochloride (S-59), in combination with UVA light, is being developed to decontaminate platelet concentrates and plasma in a blood-bank setting. S-59 is a heterocyclic psoralen compound that reacts by a three-step process with nucleic acids (NAs): (1) S-59 intercalates into the double helix; (2) upon illumination with long-wavelength ultraviolet light (UVA), it covalently attaches to a single strand, forming a monoadduct; and (3) additional illumination causes a photoreaction of the monoadduct with the second NA strand, resulting in an interstrand crosslink. The reaction occurs with the genomic material of DNA- and RNA-based viruses and occurs in genomes that are single stranded as well as double stranded. Inactivation rate is related to genome size. Large genomes such as those in leukocytes are far more susceptible to inactivation than are viruses such as hepatitis B virus (HBV), which is inactivated (> 10 5 logs) under conditions being developed for blood-bank use. The efficiency of the process is affected by a number of practical considerations such as solution components and light source. The S-59 photochemical treatment process (PCT) has been optimized for platelet concentrates as currently processed for transfusion.

Journal

Seminars in HematologyElsevier

Published: Oct 1, 2001

References

  • Photochemical inactivation of viruses and bacteria in platelet concentrates by use of a novel psoralen and long-wavelength ultraviolet light
    Lin, L; Cook, DN; Wiesehahn, GP
  • Inactivation of viruses, bacteria, protozoa and leukocytes in platelet concentrates
    Corash, L
  • Psoralens as photoactive probes of nucleic acid structure and function: Organic chemistry, photochemistry and biochemistry
    Cimino, GD; Gamper, HB; Isaacs, ST
  • Applications of psoralens as probes of nucleic acid structure and function
    Shi, Y; Lipson, SE; Chi, DY
  • Photoreaction of 8-methoxypsoralen with thymidine
    Shim, SC; Kim, YZ
  • Asymmetric recognition of psoralen interstrand crosslinks by nucleotide excision repair and the error-prone repair pathways
    Barre, FX; Asseline, U; Harel-Bellan, A

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