Functional analysis of a type 2C protein phosphatase gene from Ammopiptanthus mongolicus

Functional analysis of a type 2C protein phosphatase gene from Ammopiptanthus mongolicus In Arabidopsis and certain other plant species, the type 2C protein phosphatases (PP2Cs) of the clade A class have been demonstrated to act as negative regulators in ABA-induced stress responses, such as stomatal closure. The present study reports the identification of a PP2C ortholog from the ancient desert shrub Ammopiptanthus mongolicus (Maxim.) Cheng f. (AmPP2C), which is functionally conserved over its counterparts reported from other plant species. AmPP2C was primarily expressed in leaves, with strong transcriptional accumulation being observed in the guard cells. The expression of AmPP2C was induced in response to PEG or ABA treatments, implying the potential involvement in ABA-induced stress responses. The GFP-tagging observation revealed that AmPP2C was predominantly localized to the nuclei and partly to the cytoplasm. Furthermore, BiFC assays demonstrated an interaction between AmPP2C and the typical protein kinase SnRK2.6 (AmOST1). Overexpression of AmPP2C in Arabidopsis significantly overcame the inhibition of seed germination by ABA. The transgenic Arabidopsis lines exhibited larger stomatal apertures and significantly reduced sensitivity to ABA-induced stomatal closure, which subsequently led to greater water loss and decreased biomass under PEG-simulated drought stress treatments. Under limited nitrogen or potassium supplements, plants overexpressing AmPP2C obtained a superior capability of nitrogen (N) and potassium (K) acquisition in the green parts. Therefore, the impairment of ABA-induced stomatal closure rendered by the function of PP2C helped to identify a potential survival strategy in plants suffering persistent drought stress via the maintenance of the necessary mineral nutrient acquisition driven by transpirational solute flow. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Gene Elsevier

Functional analysis of a type 2C protein phosphatase gene from Ammopiptanthus mongolicus

Loading next page...
 
/lp/elsevier/functional-analysis-of-a-type-2c-protein-phosphatase-gene-from-z0B01O2qH0
Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
0378-1119
eISSN
1879-0038
D.O.I.
10.1016/j.gene.2018.02.015
Publisher site
See Article on Publisher Site

Abstract

In Arabidopsis and certain other plant species, the type 2C protein phosphatases (PP2Cs) of the clade A class have been demonstrated to act as negative regulators in ABA-induced stress responses, such as stomatal closure. The present study reports the identification of a PP2C ortholog from the ancient desert shrub Ammopiptanthus mongolicus (Maxim.) Cheng f. (AmPP2C), which is functionally conserved over its counterparts reported from other plant species. AmPP2C was primarily expressed in leaves, with strong transcriptional accumulation being observed in the guard cells. The expression of AmPP2C was induced in response to PEG or ABA treatments, implying the potential involvement in ABA-induced stress responses. The GFP-tagging observation revealed that AmPP2C was predominantly localized to the nuclei and partly to the cytoplasm. Furthermore, BiFC assays demonstrated an interaction between AmPP2C and the typical protein kinase SnRK2.6 (AmOST1). Overexpression of AmPP2C in Arabidopsis significantly overcame the inhibition of seed germination by ABA. The transgenic Arabidopsis lines exhibited larger stomatal apertures and significantly reduced sensitivity to ABA-induced stomatal closure, which subsequently led to greater water loss and decreased biomass under PEG-simulated drought stress treatments. Under limited nitrogen or potassium supplements, plants overexpressing AmPP2C obtained a superior capability of nitrogen (N) and potassium (K) acquisition in the green parts. Therefore, the impairment of ABA-induced stomatal closure rendered by the function of PP2C helped to identify a potential survival strategy in plants suffering persistent drought stress via the maintenance of the necessary mineral nutrient acquisition driven by transpirational solute flow.

Journal

GeneElsevier

Published: May 5, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial