Fractionation of Proteins From Whey With Different Pilot Scale Processes

Fractionation of Proteins From Whey With Different Pilot Scale Processes Pilot scale production of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) from sweet and acid casein wheys using two heat precipitation and two chromatographic methods was studied. Using the heat precipitation methods, the soluble β-Lg was recovered easily by ultrafiltration, but the recovery of the low density α-La precipitate was difficult. In addition, with acid casein whey, significant denaturation of α-La occurred. β-Lg was selectively removed from acid casein whey with strongly basic silica and polystyrenic anion exchange resin columns by elution with 0.1 mol/L HCl or 0.33 mol/L NaCl solution. β-Lg eluted with HCl was highly denatured. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png LWT - Food Science and Technology Elsevier

Fractionation of Proteins From Whey With Different Pilot Scale Processes

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Ltd
ISSN
0023-6438
D.O.I.
10.1006/fstl.1996.0063
Publisher site
See Article on Publisher Site

Abstract

Pilot scale production of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) from sweet and acid casein wheys using two heat precipitation and two chromatographic methods was studied. Using the heat precipitation methods, the soluble β-Lg was recovered easily by ultrafiltration, but the recovery of the low density α-La precipitate was difficult. In addition, with acid casein whey, significant denaturation of α-La occurred. β-Lg was selectively removed from acid casein whey with strongly basic silica and polystyrenic anion exchange resin columns by elution with 0.1 mol/L HCl or 0.33 mol/L NaCl solution. β-Lg eluted with HCl was highly denatured.

Journal

LWT - Food Science and TechnologyElsevier

Published: Jan 1, 1996

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