Colloids and Surfaces A 538 (2018) 297–303 Contents lists available at ScienceDirect Colloids and Surfaces A journal homepage: www.elsevier.com/locate/colsurfa Research Paper Formation of supported lipid bilayers on porous polymeric substrates induced by hydrophobic interaction Wakana Miyashita, Daisuke Saeki, Hideto Matsuyama Center for Membrane and Film Technology, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: This study presents a technique for forming a supported lipid bilayer (SLB) on ﬂexible and porous polymeric Supported lipid bilayer substrates via a liposome rupture method induced by hydrophobic interaction. Porous polymeric substrates Porous polymeric substrate improve the mechanical strength and ﬂexibility of SLBs and allow the molecular permeation through SLBs. Three Hydrophobic interaction types of commercial polymeric microﬁltration membranes, regenerated cellulose (RC), nitrocellulose (NC) and Streptolysin O polyvinylidene ﬂuoride (PVDF), were used as porous substrates. First, the surfaces of the porous substrates were Immunostain modiﬁed with alkyl chains using octadecyltrichlorosilane or palmitoyl chloride. The degree of the alkyl chains modiﬁed on the substrates was evaluated by x-ray photoelectron spectroscopy (XPS) measurement. The modiﬁed substrates were then immersed in a liposome suspension. The SLB formation was conﬁrmed by evaluating
Colloids and Surfaces A: Physicochemical and Engineering Aspects – Elsevier
Published: Feb 5, 2018
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