Article history: In this study we describe a novel polymer, mPPS-FA, synthesized as a potential gene transfer vector. To Received 18 July 2011 complete mPPS-FA, folic acid was conjugated to a backbone (named mPPS) consisting of a copolymer Received in revised form 1 of methyl PEG-2000, PEI-600, and sebacoyl chloride. H NMR, FT-IR, and UV spectroscopy were used to 14 December 2011 characterize the structure of mPPS-FA. It was revealed that mPPS-FA holds the ability to bind plasmid DNA Accepted 7 January 2012 yielding positively charged particles (polyplexes). Dynamic light scattering (DLS) and TEM techniques Available online 14 January 2012 were used to study the size and morphology of the formed mPPS-FA/DNA nanocomplexes. The mPPS- FA/DNA nanoparticles exhibited low cytotoxicity as transfection of B16-F0, U87MG, CHO-1, and Ho-8910 Keywords: cells produced >80% viability indicating low cytotoxicity of the polymer. The ability of mPPS-FA to deliver Polymer EGFP plasmid to melanoma B16-F0, U87, CHO-1, Ho-8910, and A549 cells was investigated in vitro as Polyethylenimine TM compared to the lipid-based transfection agent Lipofectamine 2000 and Linear PEI 22kDa (L-PEI 22kDa). Tumor gene delivery PEG We found that mPPS-FA/DNA complexes yielded the highest GFP transfection efﬁciency in B16-F0, U87, CHO-1, and Ho-8910
International Journal of Pharmaceutics – Elsevier
Published: Apr 15, 2012
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