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allergen present in cow's milk. It can induce allergic reactions even at the low concentration existing in hypoallergenic formulas based on hydrolyzed cowâs milk proteins (generally lower than lM ...
it is necessary the use of fluorescence tags. This fact hindered the potential sensitivity of LIF detection due to the difficulty of labeling analytes at extremely low concentrations . In the context of micro ...
, and are as low as 5 nM for BSA. Satisfactory linear relationships between peak height (or peak area) and protein concentration were obtained by CE‐LIF for this on‐column labeling method with Red‐3 and Red‐1c. ...
electrokinetic capillary electrophoresis (MEKC) with laser-induced fluorescence (LIF) detection. The conditions for derivatization and separation of the phosphoamino acids, including pH, concentration ...
for the derivatization of nonfluorescent baclofen. HS‐β‐CD (2%) containing 50 mM borate buffer at pH 9.5 was chosen as the optimal running electrolyte and applied to the analysis of baclofen enantiomers in human plasma ...
lipophilic contaminants, ISP is derivatized by pre-column with 9-fluorenylmethyl chloroformate for fluorescence detection. Chromatographic separations are achieved using a C18 column and mobile phase ...
with sodium borohydride to reduce the disulfide bonds, and the liberated thiols were derivatized with monobromobimane. The derivatized sample, still containing the plasma proteins , was injected onto a strong ...
Hydrophobic-interaction chromatography coupled on-line with chemical-vapor-generation atomic- fluorescence spectrometry (HIC–CVGAFS), optimized recently for the analysis of thiol-containing proteins ...
with silver staining. In this method, protein molecules are derivatized with a fluorescence reagent prior to analysis . LIF detection of the covalently attached fluorophore enables accurate quantitation of low ...
in an alkaline medium (13, 31–33). Accordingly, derivatization of morphine on-line into a highly fluorescent product before performing analysis in biological fluids is needed. For this type of derivatization ...
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