Article history: The aim of the present work was to investigate the possibility of using femtosecond laser surface textur- Received 30 July 2015 ing as a method to reduce the colonization of Grade 2 Titanium alloy surfaces by Staphylococcus aureus Received in revised form 13 October 2015 and the subsequent formation of bioﬁlm. The laser treatments were carried out with a Yb:KYW chirped- Accepted 16 October 2015 pulse-regenerative ampliﬁcation laser system with a central wavelength of 1030 nm and a pulse duration Available online 9 November 2015 of 500 fs. Two types of surface textures, consisting of laser-induced periodic surface structures (LIPSS) and nanopillars, were produced. The topography, chemical composition and phase constitution of these Keywords: surfaces were investigated by atomic force microscopy, scanning electron microscopy, X-ray photoelec- Femtosecond lasers tron spectroscopy, micro-Raman spectroscopy, and X-ray diffraction. Surface wettability was assessed by Laser surface texturing the sessile drop method using water and diiodomethane as testing liquids. The response of S. aureus put Dental and orthopedic implants into contact with the laser treated surfaces in controlled conditions was investigated by epiﬂuorescence Wettability microscopy and scanning electron microscopy 48 h after cell seeding. The results achieved show that the Bacteria adhesion
Applied Surface Science – Elsevier
Published: Jan 1, 2016
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